Department of Zoology, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Department of Physiology, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Trop Anim Health Prod. 2022 Nov 7;54(6):377. doi: 10.1007/s11250-022-03377-3.
The present study has been designed to devise a pen-side hematological formula for estimation of hemoglobin (Hb) from packed cell volume (PCV) in Cholistani breed of cattle being reared under pastoralism in Cholistan desert, Pakistan. It also aims to validate the soundness of rule of calculating Hb concentration as one-third of PCV and vice versa as being used in human medicine. Cholistani cattle (n = 364) were bled for PCV determination (microhematocrit) method and Hb estimation (hematology analyzer) (HbD) as well as through calculation being one-third of PCV (HbC). The independent-sample t-test was implied for deducing difference between HbD and HbC, and between HbD and corrected Hb (CHb) for all study groups. The CHb was deduced through regression prediction equation attained from linear regression model. Scatter-plots were drawn and linear regression was carried out between various studied hematological attributes. Significantly (P ≤ 0.01) positive correlation coefficient was noticed for all study groups being highest for female adult stock (r = 0.893; adjusted R-square = 0.79) between HbD and PCV and between HbD and HbC. The regression equation for overall results attained, i.e., Hb = 0.13 (PCV) + 6.3 was used to deduce CHb. A non-significant (P ≥ 0.05) difference was noticed between HbD and CHb. In conclusion, a convention of human clinical medicine that Hb concentration is third of PCV and vice versa cannot be implied for Cholistani cattle. A different equation, i.e., Hb (g/dL) = 0.13 (PCV) + 6.3 may provide reliable results for Hb estimation from the PCV in cattle.
本研究旨在为巴基斯坦乔利斯坦沙漠地区牧民饲养的乔利斯坦牛设计一种笔侧血液学公式，用于从红细胞压积（PCV）估算血红蛋白（Hb）。它还旨在验证在人类医学中使用的计算 Hb 浓度为 PCV 的三分之一和反之亦然的规则的合理性。对 364 头乔利斯坦牛进行采血，用于 PCV 测定（微量血球压积）法和 Hb 估计（血液分析仪）（HbD）以及通过计算为 PCV 的三分之一（HbC）。对所有研究组，采用独立样本 t 检验推断 HbD 与 HbC 之间以及 HbD 与校正 Hb（CHb）之间的差异。通过从线性回归模型获得的回归预测方程来推断 CHb。绘制散点图并进行各种研究血液学属性之间的线性回归。所有研究组均观察到显著（P≤0.01）正相关系数，雌性成年牛最高（r=0.893；调整 R 平方=0.79），HbD 与 PCV 之间以及 HbD 与 HbC 之间。得出的总体结果的回归方程，即 Hb=0.13（PCV）+6.3，用于推断 CHb。HbD 与 CHb 之间无显著差异（P≥0.05）。总之，不能将人类临床医学中 Hb 浓度为 PCV 的三分之一和反之亦然的惯例应用于乔利斯坦牛。不同的方程，即 Hb（g/dL）=0.13（PCV）+6.3，可能为从牛的 PCV 估算 Hb 提供可靠的结果。
