Li Peipei, Ma Qian, Qu Chengxin, Zhu Shuliang, Zhao Kunkun, Ma Xingli, Li Zhongfeng, Zhang Xingguo, Gong Fangping, Yin Dongmei
Henan Agricultural University, College of Agronomy & Center for Crop Genome Engineering, Henan Agricultural University, Henan, China, Zhengzhou, Henan Province, China.
PeerJ. 2021 Oct 21;9:e12319. doi: 10.7717/peerj.12319. eCollection 2021.
Auxin response factors (ARFs) are transcription factors that regulate the expression of auxin response genes, and have important functions in plant growth and development. In this study, available genome data for peanut ( L.) were used to identify genes. In total, 61 and 23 were divided into six groups (I-VI). Molecular structural analysis revealed that the protein members of AhARF contain at least two domains, the B3 domain and the Auxin-resp domain, and that some have a C-terminal dimerisation domain. Screening of the transcriptome data of 22 tissues of cv. Tifrunner in a public database showed high expression levels of and . was expressed more highly in the stem and branch than in the root and leaf of the wild species () and cultivated species H103. After treatment with exogenous auxin (NAA), the expression of was inhibited, and this inhibition was greater in than in H103. The transcriptome map revealed that the expression of was higher in the larger pods of H8107 and ZP06 than in the medium pods of H103 and small pods of . Moreover, -5 was proven to be localised in the nucleus, consistent with the location of . These results suggest that may play an important role in pod development in peanut.
生长素响应因子（ARFs）是调节生长素响应基因表达的转录因子，在植物生长发育中具有重要功能。在本研究中，利用花生（ ）的可用基因组数据来鉴定 基因。总共，61个 和23个 被分为六组（I - VI）。分子结构分析表明，AhARF的蛋白质成员至少包含两个结构域，即B3结构域和生长素响应结构域，并且一些成员具有C端二聚化结构域。在公共数据库中对栽培种Tifrunner的22个组织的转录组数据进行筛选，结果显示 和 的表达水平较高。 在野生种（ ）和栽培种H103的茎和枝中的表达高于根和叶。用外源生长素（NAA）处理后， 的表达受到抑制，并且这种抑制在 中比在H103中更明显。转录组图谱显示， 在H8107和ZP06的大荚果中的表达高于H103的中荚果和 的小荚果。此外， - 5被证明定位于细胞核，与 的定位一致。这些结果表明， 可能在花生荚果发育中起重要作用。
