从大鼠甲状旁腺克隆的功能性上皮细胞系。
Functional epithelial cell line cloned from rat parathyroid glands.
作者信息
Sakaguchi K, Santora A, Zimering M, Curcio F, Aurbach G D, Brandi M L
出版信息
Proc Natl Acad Sci U S A. 1987 May;84(10):3269-73. doi: 10.1073/pnas.84.10.3269.
Abstract
Primary cultures of rat parathyroid cells were developed in medium containing 5% calf serum, 1% Nutridoma-SP (a serum-free medium supplement from Boehringer Mannheim), and 0.7 mM calcium. The PT-r strain was purified by successive colony isolations and maintained differentiated characteristics (secretion of bioactive and radioimmunoactive parathyroid hormone into the culture medium, sensitivity to calcium regulation, and modulation by secretin) for 7 months in continuous culture. These cultured cells are epithelioid, display diploid chromosome numbers, and do not show a transformed phenotype. There has been no decrease in the rate of cell division or decline in parathyroid hormone secretion since the cell line was established. This clonal cell line provides an important system for further studies on the biology of the parathyroid cell.
摘要
大鼠甲状旁腺细胞的原代培养是在含有5%小牛血清、1% Nutridoma-SP（一种来自勃林格殷格翰的无血清培养基补充剂）和0.7 mM钙的培养基中进行的。PT-r细胞株通过连续的集落分离进行纯化，并在连续培养7个月的过程中保持分化特征（向培养基中分泌生物活性和放射免疫活性甲状旁腺激素、对钙调节的敏感性以及促胰液素的调节作用）。这些培养的细胞呈上皮样，具有二倍体染色体数，且未表现出转化表型。自细胞系建立以来，细胞分裂速率没有下降，甲状旁腺激素分泌也没有减少。这个克隆细胞系为甲状旁腺细胞生物学的进一步研究提供了一个重要的系统。
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