Al-Griw Mohamed A, Marwan Zohour M, Hdud Ismail M, Shaibi Taher
Department of Histology and Genetics, Faculty of Medicine, University of Tripoli, Tripoli, Libya.
Department of Zoology, Faculty of Sciences, University of Tripoli, Tripoli, Libya.
Open Vet J. 2021 Jul-Sep;11(3):407-417. doi: 10.5455/OVJ.2021.v11.i3.12. Epub 2021 Aug 12.
During early development, environmental compounds can induce adult onset diseases and disrupt the circulating vitamin D (VitD) levels.
This study aimed to examine the protective role of VitD against the adverse effects of BPA on male and female mice.
A total of 60 male and female Swiss Albino mice (3 weeks old) were randomly divided into 5 groups; each consisted of 12 mice (6 males and 6 females) and was treated as follows: Group I received no treatment (sham control); Group II, sterile corn oil only (vehicle control); Group III, BPA (400 μg/kg); Group IV, VitD (2,195 IU/kg); and Group V, BPA + VitD. At 10.5 weeks, the animals were sacrificed to conduct histological examinations.
BPA-exposed mice were found to have neurobehavioral abnormalities, heart, kidney, and lung diseases with increased apoptotic indices in both sexes. On the other hand, the treatment of BPA mice with VitD altered this scenario with modulated motor activity, enhanced body and organ weights, and preserved the heart, kidney, and lung architecture, alongside a decreased percent apoptotic index.
Our findings illustrate that VitD protects mice against BPA-induced heart, kidney, and lung abnormalities.
在早期发育过程中，环境化合物可诱发成年期疾病并扰乱循环维生素D（VitD）水平。
本研究旨在探讨VitD对双酚A（BPA）对雄性和雌性小鼠不良影响的保护作用。
将60只3周龄的瑞士白化小鼠（雌雄各半）随机分为5组；每组12只小鼠（6只雄性和6只雌性），并进行如下处理：第一组不做处理（假对照组）；第二组仅给予无菌玉米油（溶剂对照组）；第三组给予BPA（400μg/kg）；第四组给予VitD（2195IU/kg）；第五组给予BPA+VitD。在10.5周时，处死动物进行组织学检查。
发现暴露于BPA的小鼠存在神经行为异常以及心脏、肾脏和肺部疾病，且两性的凋亡指数均增加。另一方面，用VitD治疗BPA小鼠改变了这种情况，其运动活动得到调节，体重和器官重量增加，心脏、肾脏和肺部结构得以保留，同时凋亡指数百分比降低。
我们的研究结果表明，VitD可保护小鼠免受BPA诱导的心脏、肾脏和肺部异常。
