Department of Life Sciences, Imperial College, London, United Kingdom.
Université Côte d'Azur, CNRS, Inserm, IBV, Nice, France.
Front Cell Infect Microbiol. 2021 Oct 14;11:733094. doi: 10.3389/fcimb.2021.733094. eCollection 2021.
Oomycetes are a group of eukaryotic organisms that includes many important pathogens of animals and plants. Within this group, the genus is characterised by the presence of specialised gun cells carrying a harpoon-like infection apparatus. While several pathogens have been morphologically described, there are currently no host systems developed to study the infection process or host responses in the lab. In this study, we report that species are potent natural pathogens of nematodes. Using electron microscopy, we characterise the infection process in and demonstrate that the oomycete causes excessive tissue degradation upon entry in the body cavity, whilst leaving the host cuticle intact. We also report that the host transcriptional response to infection shares similarities with the response against the oomycete , a key example of which is the induction of genes in the hypodermis. We demonstrate that this shared feature of the host response can be mounted by pathogen detection without any infection, as previously shown for . These results highlight similarities in the nematode immune response to natural infection by phylogenetically distinct oomycetes.
卵菌是一类真核生物，其中包括许多动植物的重要病原体。在这个类群中，属的特征是存在携带鱼叉状侵染装置的专门枪细胞。虽然已经对几种病原体进行了形态描述，但目前还没有开发出用于在实验室中研究侵染过程或宿主反应的宿主系统。在这项研究中，我们报告称 物种是线虫的有力天然病原体。使用电子显微镜，我们对 的侵染过程进行了表征，并证实该卵菌在进入体腔时会导致过度的组织降解，同时保持宿主表皮完整。我们还报告说，宿主对 的转录反应与对卵菌 的反应有相似之处，其中一个关键例子是在真皮层中诱导 基因的表达。我们证明，如先前针对 所显示的那样，这种宿主反应的共同特征可以通过病原体检测来引发，而无需任何感染。这些结果突出了线虫对天然感染的免疫反应与系统发育上不同的卵菌之间的相似性。
