Chen Lifeng, Hou Jing, You Bingbing, Song Feifei, Tu Xinyi, Cheng Xiaodong
Department of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Department of Gynecology, Zhejiang Provincial People's Hospital, Hangzhou, China.
Front Cell Dev Biol. 2021 Oct 14;9:728061. doi: 10.3389/fcell.2021.728061. eCollection 2021.
The present study evaluates the value of mitochondrial antiviral signaling (MAVS) expression as a potential diagnostic biomarker and therapeutic target for ovarian cancer (OC) and analyses the underlying biological mechanism in this pathology. First, the association between MAVS expression determined by immunohistochemical (IHC) and clinical characteristics was systematically investigated. Overexpression of MAVS was associated with advanced clinical factors and poor survival of OC patients. Second, bioinformatics analyses, namely, gene expression, mutation analysis, gene set variation analysis (GSVA), gene set enrichment analysis (GSEA), and weighted gene co-expression network analysis (WGCNA), were performed to evaluate the potential biological functions of MAVS in OC. The results showed that MAVS may play a critical role in immune cell infiltration. CIBERSORT was applied to assess the infiltration of immune cells in OC. CD8+ T cells, γδT cells, and eosinophils had significantly negative correlations with MAVS expression. Finally, sensitivity analysis found that patients with high MAVS expression were predicted to be significantly less responsive to cisplatin and paclitaxel. In conclusion, these findings suggested that MAVS influences biological behavior by regulating the immune response and that it can be used as a predictive marker for poor prognosis in OC.
本研究评估线粒体抗病毒信号（MAVS）表达作为卵巢癌（OC）潜在诊断生物标志物和治疗靶点的价值，并分析该病理过程中的潜在生物学机制。首先，系统研究了免疫组织化学（IHC）测定的MAVS表达与临床特征之间的关联。MAVS过表达与OC患者的晚期临床因素和不良生存相关。其次，进行了生物信息学分析，即基因表达、突变分析、基因集变异分析（GSVA）、基因集富集分析（GSEA）和加权基因共表达网络分析（WGCNA），以评估MAVS在OC中的潜在生物学功能。结果表明，MAVS可能在免疫细胞浸润中起关键作用。应用CIBERSORT评估OC中免疫细胞的浸润情况。CD8 + T细胞、γδT细胞和嗜酸性粒细胞与MAVS表达呈显著负相关。最后，敏感性分析发现，MAVS高表达的患者预计对顺铂和紫杉醇的反应显著较低。总之，这些发现表明MAVS通过调节免疫反应影响生物学行为，并且它可以用作OC预后不良的预测标志物。
