Xiao Shuzhen, Chen Tianchi, Wang Hairu, Zeng Qian, Chen Qing, Yang Zhitao, Han Lizhong, Chen Erzhen
Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Front Med (Lausanne). 2021 Oct 13;8:754944. doi: 10.3389/fmed.2021.754944. eCollection 2021.
Bloodstream infections (BSIs) are recognized as important nosocomial infections. is one of the major causes of bacteremia. This retrospective study focused on drug susceptibility and molecular epidemiology of isolated from intensive care unit (ICU) patients with BSI in Shanghai, China. Consecutive isolates were collected from ICU patients. Antibiotic susceptibility testing was conducted by the broth microdilution method. PCR was performed to detect antimicrobial resistance genes. We also completed multilocus sequence typing (MLST) and GoeBURST was used to analyze the result of MLST. A total of 78 isolates were enrolled. from ICU-BSIs were highly resistant to almost all common antibiotics. The most frequent resistance determinants responsible for extended-spectrum β-lactamase (ESBL) producers were , , and . KPC was the only enzyme, which was detected by the carbapenemase producers. The most principal sequence types (STs) were ST11, ST15, and ST23. This study presents for the first time the antibiotic resistance phenotype and molecular epidemiology of isolated from ICU patients with BSIs in Shanghai. ICU-BSI is characteristic of a high resistance rate. The occurrence of the KPC-2 enzyme may result from nosocomial clonal dissemination of ST11 .
血流感染（BSIs）被认为是重要的医院感染。是菌血症的主要原因之一。这项回顾性研究聚焦于从中国上海重症监护病房（ICU）患有血流感染的患者中分离出的[细菌名称未给出]的药敏性和分子流行病学。连续从ICU患者中收集[细菌名称未给出]分离株。采用肉汤微量稀释法进行抗生素敏感性测试。进行聚合酶链反应（PCR）以检测抗菌耐药基因。我们还完成了多位点序列分型（MLST），并使用GoeBURST分析MLST结果。共纳入78株[细菌名称未给出]分离株。来自ICU血流感染的[细菌名称未给出]对几乎所有常见抗生素都具有高度耐药性。导致产超广谱β-内酰胺酶（ESBL）的最常见耐药决定因素是[具体因素未给出]、[具体因素未给出]和[具体因素未给出]。KPC是碳青霉烯酶产生菌检测到的唯一酶。最主要的序列类型（STs）是ST11、ST15和ST23。本研究首次呈现了从上海ICU血流感染患者中分离出的[细菌名称未给出]的抗生素耐药表型和分子流行病学。ICU血流感染[细菌名称未给出]的特点是耐药率高。KPC-2酶的出现可能源于ST11的医院内克隆传播。
