Ewers Christa, Stamm Ivonne, Pfeifer Yvonne, Wieler Lothar H, Kopp Peter A, Schønning K, Prenger-Berninghoff Ellen, Scheufen Sandra, Stolle Inka, Günther Sebastian, Bethe Astrid
Institute of Hygiene and Infectious Diseases of Animals, Justus-Liebig-Universität Giessen, Giessen, Germany
Vet Med Labor GmbH, Division of IDEXX Laboratories, Ludwigsburg, Germany.
J Antimicrob Chemother. 2014 Oct;69(10):2676-80. doi: 10.1093/jac/dku217. Epub 2014 Jun 27.
To investigate the clinical relevance and molecular epidemiology of extended-spectrum β-lactamase (ESBL)-producing Klebsiella species in animals.
Antimicrobial susceptibilities and presence of ESBLs were examined among Klebsiella spp. (n = 1519) from clinical samples (>1200 senders from Germany and other European countries) mainly from companion animals and horses from October 2008 to March 2010. Multilocus sequence typing (MLST) and PFGE were performed including human isolates for comparative purposes.
The overall ESBL rate was 8% for Klebsiella pneumoniae subsp. pneumoniae. Most K. pneumoniae subsp. pneumoniae ESBL producers were isolated from soft tissue infections (29.3%) and urinary tract infections (14.9%). The major ESBL type was CTX-M-15 (85.4%), located on different plasmid scaffolds (HI2, I1, FIA, FIB, FII, A/C, R and N). Other ESBL genes, such as bla(CTX-M-1) (5.6%), bla(CTX-M-3), bla(CTX-M-9), bla(SHV-2) and bla(SHV-12) (1.1% each), were also detected. Additional resistances, e.g. to fluoroquinolones (89.9%), were frequently present. ST15-CTX-M-15, a clonal group that recently emerged in humans, accounted for 75.8% of the strains analysed by MLST and there was evidence for nosocomial events in five veterinary clinics. Human ST15-CTX-M-15 strains shared PFGE clusters with animal isolates, suggesting the dissemination of this clonal group between both populations.
Our data indicate a wide spread of ST15-CTX-M-15 K. pneumoniae subsp. pneumoniae, which should be considered as a zoonotic agent of high clinical relevance for humans and animals. Further research should be undertaken to unravel both microevolutionary and biological aspects probably contributing to this global success.
研究产超广谱β-内酰胺酶（ESBL）的克雷伯菌属在动物中的临床相关性及分子流行病学。
对2008年10月至2010年3月期间从主要来自伴侣动物和马的临床样本（来自德国和其他欧洲国家的1200多个送检者，共1519株克雷伯菌属）检测抗菌药物敏感性及ESBLs的存在情况。进行多位点序列分型（MLST）和脉冲场凝胶电泳（PFGE），并纳入人类分离株用于比较。
肺炎克雷伯菌肺炎亚种的总体ESBL率为8%。大多数肺炎克雷伯菌肺炎亚种产ESBL菌株分离自软组织感染（29.3%）和尿路感染（14.9%）。主要的ESBL类型为CTX-M-15（85.4%），位于不同的质粒支架上（HI2、I1、FIA、FIB、FII、A/C、R和N）。还检测到其他ESBL基因，如bla(CTX-M-1)（5.6%）、bla(CTX-M-3)、bla(CTX-M-9)、bla(SHV-2)和bla(SHV-12)（各1.1%）。还经常存在其他耐药性，如对氟喹诺酮类药物的耐药性（89.9%）。ST15-CTX-M-15是最近在人类中出现的一个克隆群，占MLST分析菌株的75.8%，并且在五家兽医诊所存在医院感染事件的证据。人类ST15-CTX-M-15菌株与动物分离株共享PFGE聚类，表明该克隆群在两个群体之间传播。
我们的数据表明ST15-CTX-M-15肺炎克雷伯菌肺炎亚种广泛传播，应将其视为对人类和动物具有高度临床相关性的人畜共患病原体。应进一步开展研究以阐明可能促成这种全球传播的微观进化和生物学方面。
