Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
STAR Protoc. 2021 Oct 19;2(4):100894. doi: 10.1016/j.xpro.2021.100894. eCollection 2021 Dec 17.
NLR family CARD domain containing protein 4 (NLRC4) inflammasome activation and the associated pyroptosis are critical for protection against infection by bacterial pathogens. This protocol presents a detailed procedure to activate and measure NLRC4 inflammasome activation and pyroptosis upon infection. The techniques can be adapted to monitoring the activation of other types of inflammasomes and pathogenic stimuli. For comprehensive details on the use and execution of this protocol, please refer to Dong et al. (2021).
NLR 家族 CARD 结构域包含蛋白 4（NLRC4）炎性小体的激活及其相关的细胞焦亡对于抵抗细菌病原体的感染至关重要。本方案介绍了一种详细的程序，用于在感染后激活和测量 NLRC4 炎性小体的激活和细胞焦亡。这些技术可以适用于监测其他类型的炎性小体和病原体刺激的激活。如需详细了解本方案的使用和实施，请参考 Dong 等人（2021 年）。
