State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.
Department of Sports Medicine and Adult Reconstructive Surgery, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.
Biotechnol Bioeng. 2022 Feb;119(2):591-604. doi: 10.1002/bit.27976. Epub 2021 Nov 9.
Recent evidence shows that the curvature of porous scaffold plays a significant role in guiding tissue regeneration. However, the underlying mechanism remains controversial to date. In this study, we developed an in silico model to simulate the effect of surface curvature on the osteoconduction of scaffold implants, which comprises the primary aspects of bone regeneration. Selective laser melting was used to manufacture a titanium scaffold with channels representative of different strut curvatures for in vivo assessment. The titanium scaffold was implanted in the femur condyles of rabbits to validate the mathematical model. Simulation results suggest that the curvature affected the distribution of growth factors and subsequently induced the migration of osteoblast lineage cells and bone deposition to the locations with higher curvature. The predictions of the mathematical model are in good agreement with the in vivo assessment results, in which newly formed bone first appeared adjacent to the vertices of the major axes in elliptical channels. The mechanism of curvature-guided osteoconduction may provide a guide for the design optimization of scaffold implants to achieve enhanced bone ingrowth.
最近的证据表明，多孔支架的曲率在引导组织再生方面起着重要作用。然而，其潜在机制至今仍存在争议。在这项研究中，我们开发了一种计算机模型来模拟表面曲率对支架植入物成骨作用的影响，这包含了骨再生的主要方面。我们使用选择性激光熔化技术制造了一种具有不同支柱曲率通道的钛支架，用于体内评估。将钛支架植入兔股骨髁中以验证数学模型。模拟结果表明，曲率影响生长因子的分布，从而诱导成骨细胞系细胞的迁移和骨沉积到曲率较高的位置。数学模型的预测与体内评估结果吻合良好，其中新形成的骨首先出现在椭圆形通道的长轴顶点附近。曲率引导成骨的机制可为支架植入物的设计优化提供指导，以实现增强的骨长入。
