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可生物降解支架中持续释放甲磺酰甲烷可增强有效的骨再生。

Sustained Delivery of Methylsulfonylmethane from Biodegradable Scaffolds Enhances Efficient Bone Regeneration.

机构信息

Department of Orthopaedics, Foshan Hospital of Traditional Chinese Medicine, Foshan, 528000, People's Republic of China.

Xuzhou Central Hospital, Xuzhou, 221009, People's Republic of China.

出版信息

Int J Nanomedicine. 2022 Oct 14;17:4829-4842. doi: 10.2147/IJN.S377036. eCollection 2022.

DOI:10.2147/IJN.S377036
PMID:36246935
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9558569/
Abstract

INTRODUCTION

As a popular dietary supplement containing sulfur compound, methylsulfonylmethane (MSM) has been widely used as an alternative oral medicine to relieve joint pain, reduce inflammation and promote collagen protein synthesis. However, it is rarely used in developing bioactive scaffolds in bone tissue engineering.

METHODS

Three-dimensional (3D) hydroxyapatite/poly (lactide--glycolide) (HA/PLGA) porous scaffolds with different doping levels of MSM were prepared using the phase separation method. MSM loading efficiency, in vitro drug release as well as the biological activity of MSM-loaded scaffolds were investigated by incubating mouse pre-osteoblasts (MC3T3-E1) in the uniform and interconnected porous scaffolds.

RESULTS

Sustained release of MSM from the scaffolds was observed, and the total MSM release from 1% and 10% MSM/HA/PLGA scaffolds within 16 days was up to 64.9% and 68.2%, respectively. Cell viability, proliferation, and alkaline phosphatase (ALP) activity were significantly promoted by incorporating 0.1% of MSM in the scaffolds. In vivo bone formation ability was significantly enhanced for 1% MSM/HA/PLGA scaffolds indicated by the repair of rabbit radius defects which might be affected by a stimulated release of MSM by enzyme systems in vivo.

DISCUSSION

Finding from this study revealed that the incorporation of MSM would be effective in improving the osteogenesis activity of the HA/PLGA porous scaffolds.

摘要

简介

作为一种含有硫化合物的流行膳食补充剂，甲基磺酰甲烷（MSM）已被广泛用作替代口服药物，以缓解关节疼痛、减轻炎症和促进胶原蛋白蛋白合成。然而，它很少用于开发骨组织工程中的生物活性支架。

方法

采用相分离法制备了不同掺杂水平 MSM 的三维（3D）羟基磷灰石/聚（乳酸-共-乙醇酸）（HA/PLGA）多孔支架。通过将小鼠前成骨细胞（MC3T3-E1）在均匀且相互连接的多孔支架中孵育，研究了 MSM 负载支架的 MSM 负载效率、体外药物释放以及 MSM 负载支架的生物活性。

结果

观察到 MSM 从支架中的持续释放，在 16 天内，1%和 10%MSM/HA/PLGA 支架中总 MSM 的释放量分别达到 64.9%和 68.2%。在支架中掺入 0.1%的 MSM 可显著促进细胞活力、增殖和碱性磷酸酶（ALP）活性。1%MSM/HA/PLGA 支架的体内骨形成能力显著增强，这可能受到体内酶系统刺激 MSM 释放的影响，兔桡骨缺损的修复表明了这一点。

讨论

本研究结果表明，掺入 MSM 将有效提高 HA/PLGA 多孔支架的成骨活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa6f/9558569/523b1108b931/IJN-17-4829-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa6f/9558569/5e67116fff42/IJN-17-4829-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa6f/9558569/9b11dcf15f62/IJN-17-4829-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa6f/9558569/b03e1cb0609d/IJN-17-4829-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa6f/9558569/e50c8fdc84de/IJN-17-4829-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa6f/9558569/cb3c843177ff/IJN-17-4829-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa6f/9558569/151fce0eb808/IJN-17-4829-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa6f/9558569/523b1108b931/IJN-17-4829-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa6f/9558569/5e67116fff42/IJN-17-4829-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa6f/9558569/9b11dcf15f62/IJN-17-4829-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa6f/9558569/b03e1cb0609d/IJN-17-4829-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa6f/9558569/e50c8fdc84de/IJN-17-4829-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa6f/9558569/cb3c843177ff/IJN-17-4829-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa6f/9558569/151fce0eb808/IJN-17-4829-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa6f/9558569/523b1108b931/IJN-17-4829-g0007.jpg

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