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采用人羊膜间充质基质细胞治疗宫腔粘连，通过 Notch 信号促进子宫内膜再生和修复。

Management of intrauterine adhesions using human amniotic mesenchymal stromal cells to promote endometrial regeneration and repair through Notch signalling.

机构信息

Department of Obstetrics and Gynecology, University-Town Hospital of Chongqing Medical University, Chongqing, China.

出版信息

J Cell Mol Med. 2021 Dec;25(23):11002-11015. doi: 10.1111/jcmm.17023. Epub 2021 Nov 1.

DOI:10.1111/jcmm.17023
PMID:34724320
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8642679/
Abstract

Intrauterine adhesions (IUAs) severely hamper women's reproductive functions. Human amniotic mesenchymal stromal cell (hAMSC) transplantation is effective in treating IUAs. Here, we examined the function of Notch signalling in IUA treatment with hAMSC transplantation. Forty-five Sprague-Dawley female rats were randomly divided into the sham operation, IUA, IUA + E2, IUA + hAMSCs and IUA + hAMSCs + E2 groups. After IUA induction in the rats, hAMSCs promoted endometrial regeneration and repair via differentiation into endometrial epithelial cells. In all groups, the expression of key proteins in Notch signalling was detected in the uterus by immunohistochemistry. The results indicated Notch signalling activation in the hAMSCs and hAMSCs + E2 groups. We could also induce hAMSC differentiation to generate endometrial epithelial cells in vitro. Furthermore, the inhibition of Notch signalling using the AdR-dnNotch1 vector suppressed hAMSC differentiation (assessed by epithelial and mesenchymal marker levels), whereas its activation using the AdR-Jagged1 vector increased differentiation. The above findings indicate Notch signalling mediates the differentiation of hAMSCs into endometrial epithelial cells, thus promoting endometrial regeneration and repair; Notch signalling could have an important function in IUA treatment.

摘要

宫腔粘连（IUAs）严重影响女性生殖功能。人羊膜间充质基质细胞（hAMSC）移植在治疗 IUAs 方面有效。在这里，我们研究了 Notch 信号通路在 hAMSC 移植治疗 IUAs 中的作用。45 只 Sprague-Dawley 雌性大鼠被随机分为假手术、IUA、IUA+E2、IUA+hAMSCs 和 IUA+hAMSCs+E2 组。在大鼠诱导 IUA 后，hAMSCs 通过分化为子宫内膜上皮细胞促进子宫内膜再生和修复。在所有组中，通过免疫组织化学检测子宫中 Notch 信号通路的关键蛋白表达。结果表明 hAMSCs 和 hAMSCs+E2 组中 Notch 信号通路被激活。我们还可以在体外诱导 hAMSC 分化为子宫内膜上皮细胞。此外，使用 AdR-dnNotch1 载体抑制 Notch 信号通路会抑制 hAMSC 分化（通过上皮和间充质标志物水平评估），而使用 AdR-Jagged1 载体激活 Notch 信号通路会增加分化。上述发现表明 Notch 信号通路介导 hAMSC 分化为子宫内膜上皮细胞，从而促进子宫内膜再生和修复； Notch 信号通路在 IUAs 治疗中可能具有重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a05/8642679/3282ab538efc/JCMM-25-11002-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a05/8642679/48a5c6ca36d0/JCMM-25-11002-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a05/8642679/72203b9faa0f/JCMM-25-11002-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a05/8642679/fab6e736579e/JCMM-25-11002-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a05/8642679/daa786f8912d/JCMM-25-11002-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a05/8642679/031f68f0c49b/JCMM-25-11002-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a05/8642679/3282ab538efc/JCMM-25-11002-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a05/8642679/48a5c6ca36d0/JCMM-25-11002-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a05/8642679/72203b9faa0f/JCMM-25-11002-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a05/8642679/fab6e736579e/JCMM-25-11002-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a05/8642679/daa786f8912d/JCMM-25-11002-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a05/8642679/031f68f0c49b/JCMM-25-11002-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a05/8642679/3282ab538efc/JCMM-25-11002-g001.jpg

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Mol Cell Biochem. 2021 Apr;476(4):1717-1727. doi: 10.1007/s11010-020-04012-1. Epub 2021 Jan 11.
3
Advances in biomaterials and regenerative medicine for primary ovarian insufficiency therapy.
人脐带间充质干细胞衍生的细胞外囊泡和生物材料组装体在子宫内膜损伤治疗中的研究进展
World J Stem Cells. 2025 Jan 26;17(1):97905. doi: 10.4252/wjsc.v17.i1.97905.
4
Long-term therapeutic effects of allogeneic mesenchymal stem cell transplantation for intrauterine adhesions.同种异体间充质干细胞移植治疗宫腔粘连的长期疗效
Stem Cell Res Ther. 2024 Dec 23;15(1):499. doi: 10.1186/s13287-024-04100-9.
5
Advances in Nanomedicine and Biomaterials for Endometrial Regeneration: A Comprehensive Review.纳米医学和生物材料在子宫内膜再生中的进展：全面综述。
Int J Nanomedicine. 2024 Aug 14;19:8285-8308. doi: 10.2147/IJN.S473259. eCollection 2024.
6
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Front Transplant. 2023 May 5;2:1152068. doi: 10.3389/frtra.2023.1152068. eCollection 2023.
7
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7
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