Centre National de Recherche et de Formation sur le Paludisme (CNRFP), Ministère de la Santé, Ouagadougou, Burkina Faso.
Department of Medical Microbiology and Radboud Center for Infectious Diseases, Radboud University Medical Centre, Nijmegen, The Netherlands.
Am J Trop Med Hyg. 2021 Nov 1;106(2):664-666. doi: 10.4269/ajtmh.21-0493.
We evaluated the detectability of Plasmodium falciparum clones when assessed on 3 consecutive days in incident and chronic infections in naturally exposed children living in an area of intense malaria transmission in Burkina Faso. The median number of clones by merozoite surface protein 2 (MSP2) genotyping was 3 (interquartile range [IQR] 2-5) in incident infections compared with 6 (IQR 4-8) in chronic infections (P < 0.0001). When all clones detected on days 1-3 were considered as true complexity of infection, sampling on day 1 detected only 69.4% (109/157) or 68.3% (228/334) of all clones in incident and chronic infections, respectively. Our findings demonstrate that a large proportion of clones are missed by single time-point sampling. In addition, because of the high complexity of infection early in incident infections, our data suggest many infections may be caused by genetically complex inocula.
我们评估了当在布基纳法索疟疾传播严重地区自然暴露的儿童中连续 3 天评估时，急性和慢性感染中疟原虫裂殖子表面蛋白 2 (MSP2) 基因分型时恶性疟原虫克隆的检测能力。与慢性感染相比，急性感染中通过 MSP2 基因分型检测到的克隆中位数为 3（四分位距 [IQR] 2-5），而慢性感染中为 6（IQR 4-8）（P < 0.0001）。当将第 1-3 天检测到的所有克隆均视为真正的感染复杂性时，第 1 天的采样仅分别检测到急性和慢性感染中所有克隆的 69.4%（109/157）或 68.3%（228/334）（P < 0.0001）。我们的研究结果表明，单次采样会遗漏很大一部分克隆。此外，由于急性感染早期感染的复杂性很高，我们的数据表明，许多感染可能是由遗传上复杂的接种物引起的。
