Brox L W, Belch A, Pollock E, He X X, De Braekeleer M, Lin C C
Cancer Genet Cytogenet. 1987 Jul;27(1):135-44. doi: 10.1016/0165-4608(87)90268-8.
Two new human plasma cell lines designated as ACB-885 and ACB-1085 have been established from a 39-year-old patient with multiple myeloma. These cell lines have definitive plasma cell features by morphologic examination, and essentially all of the cells are positive for cytoplasmic IgG kappa immunoglobulin. These cells are negative for standard T-cell surface markers and mature B-cell markers, such as B1, B2, and HLA-DR, but are strongly positive for the antigen defined by OKT-10. The cells are negative for Epstein-Barr virus. The cell lines have a doubling time of 30-35 hours and a growth fraction approaching 100%. Cytogenetic analysis showed a 2n chromosome number of 45-46 with very similar karyotypic abnormalities in both the plasma cell lines and the original tumor material. One of the chromosomes in each of the pairs of chromosomes number #1, #2, #6, #7, #8, #10, #12, #13, and #22 were consistently missing. These were replaced by eight marker chromosomes that resulted from chromosomal rearrangements involving mainly these missing chromosomes. Almost all of the breakpoints occurring in the marker chromosomes were identified, and eight of these breakpoints have been reported in other studies of myeloma plasma cells. Homogeneously staining regions were observed in two marker chromosomes suggesting gene amplification in these chromosomal regions.
从一名39岁的多发性骨髓瘤患者中建立了两个新的人浆细胞系，分别命名为ACB - 885和ACB - 1085。通过形态学检查，这些细胞系具有明确的浆细胞特征，并且基本上所有细胞的细胞质IgG κ免疫球蛋白均呈阳性。这些细胞对于标准T细胞表面标志物和成熟B细胞标志物（如B1、B2和HLA - DR）呈阴性，但对于由OKT - 10定义的抗原呈强阳性。这些细胞对爱泼斯坦 - 巴尔病毒呈阴性。这些细胞系的倍增时间为30 - 35小时，生长分数接近100%。细胞遗传学分析显示，浆细胞系和原始肿瘤材料的染色体数目均为2n，为45 - 46条，核型异常非常相似。在1号、2号、6号、7号、8号、10号、12号、13号和22号染色体对中，每对中的一条染色体始终缺失。这些染色体被八条标记染色体所取代，这些标记染色体是由主要涉及这些缺失染色体的染色体重排产生的。几乎所有在标记染色体中出现的断点都已被识别，其中八个断点在其他骨髓瘤浆细胞研究中已有报道。在两条标记染色体中观察到均匀染色区，提示这些染色体区域存在基因扩增。
