Jernberg H, Zech L, Nilsson K
Department of Pathology, University of Uppsala, Sweden.
Int J Cancer. 1987 Dec 15;40(6):811-7. doi: 10.1002/ijc.2910400618.
Cell lines (U-266, U-1957, U-1996 and U-2030) established from 4 patients with multiple myeloma (MM) were analyzed cytogenetically. The cell lines represent different stages in B-cell differentiation as evidenced by ultrastructural and functional characteristics. The karyotypic pattern in 3 newly established myeloma lines was studied after a few months in culture and compared to the old myeloma cell line U-266, which was examined after 6, 7 and 8 years of continuous cultivation. Frequency of progressive numerical and structural aberrations during long-term cultivation and their correlation with alterations in growth properties were addressed. We describe the presence of a high frequency of both numerical and structural chromosomal abnormalities in the cells of all 4 myeloma lines studied. Chromosomes often associated with structural abnormalities were 1, 3, 6, 12 and 14. A 14q + marker chromosome was detected in 2 of the 4 cell lines. The breakpoints on the chromosomes participating in structural aberrations in myeloma exhibit some correlation to chromosome sites at or close to locations of mapped oncogenes. No translocations of c-myc were found. These data were further supported by Southern blot analysis (unpublished data). The extent of numerical, but not structural, aberrations correlates with the differentiation stage of the myeloma lines in that the 2 mature lines U-266 and U-1957 were both near-diploid. Multiple progressive chromosomal changes have emerged in U-266 during a period of 8 years with development of independence of feeder cells and increased growth rate. However, capacity for production of complete Ig molecules has remained stable.
对从4例多发性骨髓瘤（MM）患者中建立的细胞系（U - 266、U - 1957、U - 1996和U - 2030）进行了细胞遗传学分析。这些细胞系代表了B细胞分化的不同阶段，超微结构和功能特征证明了这一点。在培养几个月后研究了3个新建立的骨髓瘤细胞系的核型模式，并与连续培养6、7和8年后检测的旧骨髓瘤细胞系U - 266进行了比较。探讨了长期培养过程中渐进性数字和结构畸变的频率及其与生长特性改变的相关性。我们描述了在所研究的所有4个骨髓瘤细胞系的细胞中均存在高频的数字和结构染色体异常。经常与结构异常相关的染色体是1、3、6、12和14。在4个细胞系中的2个中检测到14q +标记染色体。参与骨髓瘤结构畸变的染色体上的断点与定位癌基因位置处或附近的染色体位点存在一些相关性。未发现c - myc的易位。这些数据得到了Southern印迹分析（未发表数据）的进一步支持。数字畸变的程度而非结构畸变的程度与骨髓瘤细胞系的分化阶段相关，因为2个成熟细胞系U - 266和U - 1957均接近二倍体。在8年的时间里，U - 266出现了多个渐进性染色体变化，同时出现了对饲养细胞的独立性增加和生长速率提高的情况。然而，完整Ig分子的产生能力保持稳定。
