Department of Animal Breeding, Desert Research Center, Cairo, Egypt.
Anim Biotechnol. 2023 Jun;34(3):679-685. doi: 10.1080/10495398.2021.1996387. Epub 2021 Nov 2.
The aim of the present study was to detect the gene polymorphisms in Barki sheep at a variable region covering exon 13, intron 13 and the coding sequence in exon 14 and to test the association of these polymorphisms with growth traits. 122 Barki lambs were phenotyped for various growth traits, viz., birth weight (BW), weaning weight (WW), pre-weaning daily gain in weight (ADG1), post-weaning daily gain in weight (ADG2) and marketing bodyweight (MW). The polymerase chain reaction - single-strand conformational polymorphisms (PCR-SSCP) and DNA sequencing methods were used to identify the genetic variants in the gene. The associations between the variation in gene and growth traits were tested using a general linear model. Two variants ( and with gene frequencies of 0.64 and 0.36, respectively), and three genotypes (, and with frequencies of 0.37, 0.53 and 0.10, respectively) were detected. The association of genotype was significant (<0.05) with ADG2 and MW. It is concluded that genotype might be helpful for sheep breeders to produce fast-growing lambs. However, further studies are needed in a large population to confirm the association we found.
本研究旨在检测 Barki 绵羊外显子 13、内含子 13 和外显子 14 编码序列可变区的基因多态性，并检验这些多态性与生长性状的关联性。122 只 Barki 羔羊进行了各种生长性状的表型分析，包括初生重（BW）、断奶重（WW）、断奶前日增重（ADG1）、断奶后日增重（ADG2）和上市体重（MW）。采用聚合酶链反应-单链构象多态性（PCR-SSCP）和 DNA 测序方法鉴定基因中的遗传变异。采用一般线性模型检验基因变异与生长性状的关联性。检测到两种变异（和，基因频率分别为 0.64 和 0.36）和三种基因型（、和，频率分别为 0.37、0.53 和 0.10）。基因型与 ADG2 和 MW 显著相关（<0.05）。结论是基因型可能有助于绵羊饲养者生产生长速度较快的羔羊。然而，需要在更大的群体中进行进一步的研究来证实我们发现的关联性。
