Arshad Adnan
Department of Botany, Government College University, Faisalabad, 3800, Pakistan.
Heliyon. 2021 Oct 19;7(10):e08198. doi: 10.1016/j.heliyon.2021.e08198. eCollection 2021 Oct.
Experimental studies were conducted to estimate the possible damage caused to ten rice (Oryza sativa L.) genotypes of Pakistan by tropospheric ozone. The experimental site is located at 31.4504° N and 73.1350° E, at an altitude of 184 m.a.s level with an average annual rainfall of 784 mm. A suitable and agile method was adopted to assess tolerance and susceptibility in rice genotypes at an early growth stage. Genotype Injury response, growth and biochemical parameters were measured to estimate possible effects of ozone, which was subsequently proclaimed as a criterion for ozone tolerance. Rice genotypes were subjected to ozone concentrations of 70 pbb (Current ambient) and 120 pbb (expected in near future) under a polytunnel. The findings indicated that ozone, an atmospheric pollutant, substantially harmed crop growth and metabolism, as well as inflicted a specific type of foliar injury that caused early leaf senescence. Rice genotype IR-9 followed by Punjab-Basmati and Ksk-434 appeared to be the most susceptible, whereas Basmati-515 followed by Basmati 2000 and super-Basmati were found to be Ozone-tolerant. Plant genotypes grown under elevated ozone showed 13.45% and 11.35% reduction in total root and shoot dry weight, and 25.54% and 6.6% decrease in plant leaf area and plant total length respectively compared to the control group. A significant interaction between treatment × chemical components and growth parameters was also found. The Present study confirms a direct relationship between visual response and growth as well as biochemical parameters. Declared results were statistically analyzed by using analysis of variance at confidence level of p < 0.05.
开展了实验研究，以估算对流层臭氧对巴基斯坦的10个水稻（Oryza sativa L.）基因型可能造成的损害。实验地点位于北纬31.4504°、东经73.1350°，海拔184米，平均年降雨量为784毫米。采用了一种合适且灵活的方法来评估水稻基因型在生长早期的耐受性和敏感性。测量了基因型损伤反应、生长和生化参数，以估算臭氧的可能影响，随后将其作为臭氧耐受性的标准。在塑料大棚下，使水稻基因型暴露于70 ppb（当前环境浓度）和120 ppb（预计在不久的将来）的臭氧浓度下。研究结果表明，大气污染物臭氧严重损害了作物生长和新陈代谢，并造成了一种特定类型的叶片损伤，导致叶片过早衰老。水稻基因型IR-9，其次是旁遮普香米和Ksk-434，似乎最敏感，而Basmati-515，其次是Basmati 2000和超级香米，被发现具有耐臭氧性。与对照组相比，在高浓度臭氧环境下生长的植物基因型的总根干重和地上部干重分别减少了13.45%和11.35%，植物叶面积和植株总长度分别减少了25.54%和6.6%。还发现处理×化学成分与生长参数之间存在显著相互作用。本研究证实了视觉反应与生长以及生化参数之间的直接关系。使用置信水平为p < 0.05的方差分析对公布的结果进行了统计分析。
