Department of First Aid and Emergency, Vocational School of Medical Services, Mersin University, Mersin, Turkey.
Department of Biophysics, Faculty of Medicine, Mersin University, Mersin, Turkey.
Andrologia. 2022 Feb;54(1):e14291. doi: 10.1111/and.14291. Epub 2021 Nov 3.
In recent studies, it has been reported that ion channels play an important role in cancer formation. Therefore, it is possible that the use of pharmacological agents targeting ion channels will allow the development of new strategies for cancer treatment. In this study, we investigate the effect of imipramine on Eag1 channel expression in DU145 prostate cancer cells. Culture cells were divided into 4 groups as the control, 10, 50 and 75 µM imipramine. Eag1 channel currents and conductivity were determined by whole-cell patch-clamp technique and gene expression by real time-polymerase chain reaction (RT-PCR). Current records were taken before (at 0th minute, as control) and 10 min after imipramine administration to the cells. It was observed that all three doses of imipramine significantly reduced Eag1 currents and conductivity compared with the control. However, the differences between dose groups were not significant. Similarly, Eag1 channel protein expression was found to be significantly reduced for all three doses of imipramine compared with the control group, but there was no significant difference in gene expression between dose groups. Obtained results suggested that imipramine has the potential to be used as a pharmacological agent targeting the Eag1 channel in the treatment of prostate cancer.
在最近的研究中，有报道称离子通道在癌症形成中发挥重要作用。因此，有可能使用针对离子通道的药物来开发新的癌症治疗策略。在这项研究中，我们研究了丙咪嗪对前列腺癌细胞 DU145 中 Eag1 通道表达的影响。培养细胞被分为 4 组：对照组、10、50 和 75µM 丙咪嗪。通过全细胞膜片钳技术和实时聚合酶链反应（RT-PCR）测定 Eag1 通道电流和电导率。在给予细胞丙咪嗪前（第 0 分钟，作为对照）和 10 分钟后记录电流。结果观察到，与对照组相比，所有三种剂量的丙咪嗪均显著降低 Eag1 电流和电导率。然而，剂量组之间的差异并不显著。同样，与对照组相比，所有三种剂量的丙咪嗪均显著降低 Eag1 通道蛋白表达，但剂量组之间的基因表达无显著差异。研究结果表明，丙咪嗪有可能作为一种针对 Eag1 通道的药理学药物用于治疗前列腺癌。
