Department of Pharmacology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.
Toxicology Research Center, AJA University of Medical Sciences, Tehran, Iran.
Prep Biochem Biotechnol. 2022;52(7):823-834. doi: 10.1080/10826068.2021.1998112. Epub 2021 Nov 3.
Fed-batch cultivation is a well-known type of submerged fermentation that is frequently used in manufacture of recombinant proteins and various kinds of enzymes, owing to its ability to produce products with high concentrations and high efficiency. In fed-batch culture, several issues must be considered; most of them are also presented in batch culture. However, feed flow rate calculation only corresponds to fed-batch fermentation and its value has a significant impact on productivity, efficiency, final concentration of product, formation of by-products, and viscosity of the culture. From this background, the present review article is an effort to gather the information on feeding strategies for fed-batch cultivation of , which is a well-known microorganism in the production of recombinant proteins and industrial enzymes, especially for therapeutic applications. Moreover, this review is an aid to comprehend and compare the fundamental concept of different feeding strategies and their advantages and drawbacks.
补料分批培养是一种众所周知的深层发酵类型，由于其能够生产高浓度和高效率的产品，因此常用于重组蛋白和各种酶的生产。在补料分批培养中，必须考虑几个问题；其中大多数问题也存在于分批培养中。然而，进料流速的计算仅对应于补料分批发酵，其值对生产力、效率、最终产物浓度、副产物的形成以及培养物的粘度有重大影响。有鉴于此，本文综述了用于生产重组蛋白和工业酶的知名微生物的补料分批培养的进料策略信息，特别是针对治疗应用。此外，本文综述有助于理解和比较不同进料策略的基本概念及其优缺点。
