Chongqing Normal University, College of Life Sciences, Shapingba, Chongqing, P.R. China.
Ningxia University, School of Life Sciences, Xixia, Yinchuan, Ningxia, P.R. China.
Braz J Biol. 2021 Oct 27;83:e249424. doi: 10.1590/1519-6984.249424. eCollection 2021.
Hypoxia is a prominent feature of head and neck cancer. However, the oxygen element characteristics of proteins and how they adapt to hypoxia microenvironments of head and neck cancer are still unknown. Human genome sequences and proteins expressed data of head and neck cancer were retrieved from pathology atlas of Human Protein Atlas project. Then compared the oxygen and carbon element contents between proteomes of head and neck cancer and normal oral mucosa-squamous epithelial cells, genome locations, pathways, and functional dissection associated with head and neck cancer were also studied. A total of 902 differentially expressed proteins were observed where the average oxygen content is higher than that of the lowly expressed proteins in head and neck cancer proteins. Further, the average oxygen content of the up regulated proteins was 2.54% higher than other. None of their coding genes were distributed on the Y chromosome. The up regulated proteins were enriched in endocytosis, apoptosis and regulation of actin cytoskeleton. The increased oxygen contents of the highly expressed and the up regulated proteins might be caused by frequent activity of cytoskeleton and adapted to the rapid growth and fast division of the head and neck cancer cells. The oxygen usage bias and key proteins may help us to understand the mechanisms behind head and neck cancer in targeted therapy, which lays a foundation for the application of stoichioproteomics in targeted therapy and provides promise for potential treatments for head and neck cancer.
缺氧是头颈部癌症的一个显著特征。然而，蛋白质的氧元素特征以及它们如何适应头颈部癌症的缺氧微环境仍然未知。从人类蛋白质图谱项目的病理学图谱中检索到头颈部癌症的人类基因组序列和蛋白质表达数据。然后比较了头颈部癌症蛋白质组和正常口腔黏膜鳞状上皮细胞蛋白质组之间的氧和碳元素含量，还研究了与头颈部癌症相关的基因组位置、途径和功能剖析。共观察到 902 个差异表达的蛋白质，其中头颈部癌症蛋白质中高表达的蛋白质的平均氧含量高于低表达的蛋白质。此外，上调蛋白的平均氧含量比其他蛋白高 2.54%。它们的编码基因都没有分布在 Y 染色体上。上调蛋白富集于内吞作用、细胞凋亡和肌动蛋白细胞骨架的调节。高表达和上调蛋白中氧含量的增加可能是由于细胞骨架的频繁活动以及适应头颈部癌细胞的快速生长和快速分裂。氧利用偏向和关键蛋白可能有助于我们了解靶向治疗背后的头颈部癌症机制，为靶向治疗中的稳定同位素蛋白质组学的应用奠定基础，并为头颈部癌症的潜在治疗提供了希望。
