香芹酚、百里香酚和乳链菌肽对金黄色葡萄球菌和肠炎沙门氏菌的联合作用。
Combined effect of carvacrol, thymol and nisin against Staphylococcus aureus and Salmonella Enteritidis.
机构信息
Universidade Federal do Rio Grande do Sul, Departamento de Ciências de Alimentos, Instituto de Ciência e Tecnologia de Alimentos, Av. Bento Gonçalves, 9500, Prédio 43.212, Campus do Vale, 91501-970 Porto Alegre, RS, Brazil.
Universidade Estadual do Rio Grande do Sul, Área da Vida e Meio Ambiente, Rua Alegrete, 821, Campus em Encantado, 95960-000 Encantado, RS, Brazil.
出版信息
An Acad Bras Cienc. 2021 Nov 1;93(suppl 4):e20210550. doi: 10.1590/0001-3765202120210550. eCollection 2021.
The aim of this study was to investigate the combined effect of carvacrol, thymol and nisin against Staphylococcus aureus and the combined effect of carvacrol and thymol against Salmonella Enteritidis. Minimum inhibitory concentrations (MIC) of carvacrol, thymol, and nisin for S. aureus were 200, 150 and 30 µg/mL, respectively. MIC of carvacrol and thymol for Salmonella Enteritidis was 200 µg/mL. A factorial method of independent variables was then used to study the combined effect of antimicrobials. Results showed that combinations of carvacrol-thymol-nisin (reduction of 1.2 log CFU/mL for MIC and 4.98 log CFU/mL for 2MIC), carvacrol-thymol (reduction of 1.33 log CFU/mL for 2MIC), nisin-thymol (reduction of 3.52 log CFU/mL for 2MIC) and nisin-carvacrol (reduction of 3.41 log CFU/mL for 2MIC) attained a significant inhibition of S. aureus. Similarly, there was significant reduction of Salmonella Enteritidis due to combined effect of thymol-carvacrol (reduction of 4.5 log CFU/mL for MIC and inhibition below detection limit for 2MIC). Therefore, the combinations of natural antimicrobials described in this work showed potential to be used as an additional barrier for food safety.
本研究旨在探讨香芹酚、百里香酚和乳链菌肽对金黄色葡萄球菌的联合作用，以及香芹酚和百里香酚对肠炎沙门氏菌的联合作用。香芹酚、百里香酚和乳链菌肽对金黄色葡萄球菌的最小抑菌浓度（MIC）分别为 200、150 和 30μg/mL。香芹酚和百里香酚对肠炎沙门氏菌的 MIC 为 200μg/mL。然后采用独立变量的析因法研究了抗菌剂的联合作用。结果表明，香芹酚-百里香酚-乳链菌肽（MIC 降低 1.2 对数 CFU/mL，2MIC 降低 4.98 对数 CFU/mL）、香芹酚-百里香酚（2MIC 降低 1.33 对数 CFU/mL）、乳链菌肽-百里香酚（2MIC 降低 3.52 对数 CFU/mL）和乳链菌肽-香芹酚（2MIC 降低 3.41 对数 CFU/mL）对金黄色葡萄球菌具有显著抑制作用。同样，由于百里香酚-香芹酚的联合作用，肠炎沙门氏菌的数量显著减少（MIC 降低 4.5 对数 CFU/mL，2MIC 抑制低于检测限）。因此，本研究中描述的天然抗菌剂组合具有作为食品安全附加屏障的潜力。
Zotero 插件