Department of Microbiology, College of Medicine, AL-Iraqia University, Baghdad, Iraq.
Department of Biology, College of Science for Women, University of Baghdad, Baghdad, Iraq.
J Cosmet Dermatol. 2022 Mar;21(3):1286-1291. doi: 10.1111/jocd.14541. Epub 2021 Nov 3.
Alopecia areata is considered to be an autoimmune disease characterized by T-cell response and production of cytokines around the anagen stage.
The aim of this study was to assess T cells in blood using immune biomarkers (CD3, CD4, and CD8) in patients with AA and AU compared with healthy individuals.
This study was carried out at private clinic and in the Department of Dermatology in Al Karama Teaching Hospital. The study groups included 58 patients from different geographic areas in Iraq, with ages ranging from 5 to 48 years from the end of August 2019 to the January 2020; AA patient group included 19, AU patient group included, 11 and control group included 30 with no scalp or body lesions in their disease history. All patients were clinically diagnosed to detect clinical types of disease in the scalp, in the body, and any hair-bearing area. Venous blood samples were collected from all participants where CD3 T cell, CD4 T cell, and CD8 T cell in whole blood were estimated by flow cytometry technique.
The present study has found the males are more susceptible to infection than the females in both types of disease. There was no significant difference (p > 0.05) detected for immune biomarkers (CD3, CD4, and CD8) in blood between patient groups in comparison with healthy individuals, but there was a significant decrease (p ≤ 0.05) in immune biomarkers (CD8, CD3) according to stress status in AU and in CD8 T cell between patient groups. CD4/CD8 ratio increased in alopecia patients.
In current study, There was no significant difference detected for biomarkers (CD3, CD4, and CD8) in blood between patient groups and healthy control, but significantly decrease in CD8 T cell and CD3T cell according to stress in AU, and in CD8T cell between patient groups according to stress This research may have proven one of the theories. Study of CD markers receptors and B cell receptors these will help for future studies.
斑秃被认为是一种自身免疫性疾病，其特征是在生长期周围发生 T 细胞反应和细胞因子的产生。
本研究旨在通过免疫生物标志物（CD3、CD4 和 CD8）评估斑秃和全秃患者血液中的 T 细胞与健康个体相比的情况。
本研究在伊拉克不同地区的私人诊所和 Al Karama 教学医院皮肤科进行。研究组包括 2019 年 8 月底至 2020 年 1 月期间的 58 名患者，年龄 5 至 48 岁；斑秃患者组包括 19 名，全秃患者组包括 11 名，对照组包括 30 名，他们在病史中没有头皮或身体病变。所有患者均经临床诊断以检测头皮、身体和任何有毛发的区域的疾病临床类型。从所有参与者中采集静脉血样，通过流式细胞术技术评估全血中的 CD3 T 细胞、CD4 T 细胞和 CD8 T 细胞。
本研究发现，男性比女性更容易感染两种疾病。与健康个体相比，患者组之间的血液免疫生物标志物（CD3、CD4 和 CD8）无显著差异（p＞0.05），但在 AU 中根据应激状态和 CD8 T 细胞在患者组之间均显著降低（p≤0.05）。斑秃患者的 CD4/CD8 比值增加。
在目前的研究中，患者组与健康对照组之间的血液生物标志物（CD3、CD4 和 CD8）无显著差异，但 AU 中根据应激状态的 CD8 T 细胞和 CD3T 细胞以及根据应激状态的患者组之间的 CD8T 细胞显著降低。这项研究可能已经证明了其中一种理论。研究 CD 标记物受体和 B 细胞受体将有助于未来的研究。
