Aix Marseille Université, CNRS, LNC-UMR 7291, 13344 Marseille, France.
Aix Marseille Université, CNRS, LNC-UMR 7291, 13344 Marseille, France.
Cell Rep. 2021 Nov 2;37(5):109914. doi: 10.1016/j.celrep.2021.109914.
A variety of mechanosensory neurons are involved in touch, proprioception, and pain. Many molecular components of the mechanotransduction machinery subserving these sensory modalities remain to be discovered. Here, we combine recordings of mechanosensitive (MS) currents in mechanosensory neurons with single-cell RNA sequencing. Transcriptional profiles are mapped onto previously identified sensory neuron types to identify cell-type correlates between datasets. Correlation of current signatures with single-cell transcriptomes provides a one-to-one correspondence between mechanoelectric properties and transcriptomically defined neuronal populations. Moreover, a gene-expression differential comparison provides a set of candidate genes for mechanotransduction complexes. Piezo2 is expectedly found to be enriched in rapidly adapting MS current-expressing neurons, whereas Tmem120a and Tmem150c, thought to mediate slow-type MS currents, are uniformly expressed in all mechanosensory neuron subtypes. Further knockdown experiments disqualify them as mediating MS currents in sensory neurons. This dataset constitutes an open resource to explore further the cell-type-specific determinants of mechanosensory properties.
各种机械感觉神经元参与触觉、本体感觉和疼痛。许多机械感觉机械转导机械的分子成分仍然有待发现。在这里，我们将机械敏感（MS）电流在机械感觉神经元中的记录与单细胞 RNA 测序相结合。转录谱被映射到先前鉴定的感觉神经元类型上，以确定数据集之间的细胞类型相关性。电流特征与单细胞转录组的相关性提供了机械电特性与转录定义的神经元群体之间的一一对应关系。此外，基因表达差异比较提供了一组候选机械转导复合物基因。Piezo2 预计在快速适应 MS 电流表达神经元中富集，而 Tmem120a 和 Tmem150c，被认为介导慢型 MS 电流，在所有机械感觉神经元亚型中均匀表达。进一步的敲低实验使它们失去了在感觉神经元中介导 MS 电流的能力。这个数据集构成了一个开放的资源，可以进一步探索机械感觉特性的细胞类型特异性决定因素。
