Suazo Iván, García-Mesa Yolanda, Martín-Cruces José, Cuendias Patricia, Cobo Teresa, García-Suárez Olivia, Vega José A
Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Providencia 7500912, Chile.
Grupo SINPOS, Departamento de Morfología y Biología Celular, Universidad de Oviedo, 33006 Oviedo, Spain.
Brain Sci. 2025 Mar 24;15(4):337. doi: 10.3390/brainsci15040337.
BACKGROUND/OBJECTIVES: Tentonin-3/TMEM150C is a pore-forming protein of a mechanically activated channel recently identified that typically displays rapid activation followed by slow inactivation. It has been detected in murine dorsal root ganglia, nodose ganglion baroreceptors, and muscle spindles. Nevertheless, primary sensory neurons expressing tentonin-3/TMEM150C fall into the categories of nociceptors, mechanoreceptors, and proprioceptors.
We used immunohistochemistry and image analysis (examining the size of the neuronal bodies in the dorsal root ganglia) to investigate the distribution of tentonin-3/TMEM150C in human cervical dorsal root ganglia, sensory nerve formations in the glabrous skin, especially cutaneous end-organ complexes or sensory corpuscles, and muscle spindles.
In dorsal root ganglia, 41% of neurons were tentonin-3/TMEM150C-positive, with a distribution of small (12.0%), intermediate (18.1%), and large (10.9%). In the glabrous skin, tentonin-3/TMEM150C was observed in the axon of Meissner, Pacinian, and Ruffini corpuscles as well as in the axon of the Merkel cell-axon complexes. Furthermore, tentonin-3/TMEM150C-positive axons were observed in muscle spindles. No free nerve endings displaying immunoreactivity were found.
This is the first report on the distribution of tentonin-3/TMEM150C immunoreactivity in the human peripheral somatosensory system, and although it is a brief preliminary study, it opens new perspectives for the study of this new mechano-gated ion channel.
背景/目的：Tentonin-3/TMEM150C是最近发现的一种机械激活通道的成孔蛋白，通常表现出快速激活后缓慢失活。它已在小鼠背根神经节、结状神经节压力感受器和肌梭中被检测到。然而，表达Tentonin-3/TMEM150C的初级感觉神经元可分为伤害感受器、机械感受器和本体感受器。
我们使用免疫组织化学和图像分析（检查背根神经节中神经元胞体的大小）来研究Tentonin-3/TMEM150C在人颈背根神经节、无毛皮肤的感觉神经结构，特别是皮肤终末器官复合体或感觉小体以及肌梭中的分布。
在背根神经节中，41%的神经元Tentonin-3/TMEM150C呈阳性，其分布为小（12.0%）、中（18.1%）和大（10.9%）。在无毛皮肤中，在迈斯纳小体、环层小体和鲁菲尼小体的轴突以及默克尔细胞-轴突复合体的轴突中观察到Tentonin-3/TMEM150C。此外，在肌梭中观察到Tentonin-3/TMEM150C阳性轴突。未发现显示免疫反应性的游离神经末梢。
这是关于Tentonin-3/TMEM150C免疫反应性在人外周躯体感觉系统中分布的首次报道，尽管这是一项简短的初步研究，但它为研究这种新的机械门控离子通道开辟了新的视角。
