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正常小鼠与围手术期神经认知障碍小鼠海马差异蛋白表达的比较：蛋白质组学分析。

Differential hippocampal protein expression between normal mice and mice with the perioperative neurocognitive disorder: a proteomic analysis.

机构信息

Department of Anesthesiology, Qingdao Municipal Hospital Affiliated to Qingdao University, 5 Donghai Middle Road, Qingdao, Shandong, 266071, People's Republic of China.

Department of Anesthesiology, Dalian Medical University, Dalian, Liaoning, China.

出版信息

Eur J Med Res. 2021 Nov 3;26(1):130. doi: 10.1186/s40001-021-00599-3.

DOI:10.1186/s40001-021-00599-3
PMID:34732255
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8565051/
Abstract

OBJECTIVES

To compare differential expression protein in hippocampal tissues from mice of perioperative neurocognitive disorder (PND) and normal control mice and to explore the possible mechanism of PND.

METHODS

Mice were randomly divided into a PND group (n = 9) and a control group (n = 9).The mice in the PND group were treated with open tibial fracture with intramedullary fixation under isoflurane anesthesia, while the mice in the control group received pure oxygen without surgery. The cognitive functions of the two groups were examined using Morris water maze experiment, Open field test and Fear conditioning test. The protein expression of the hippocampus of mice was analyzed by high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to explore the principal functions of dysregulated proteins.

RESULTS

A total of 21 proteins were differentially expressed between PND and control mice on days 1, 3, and 7 after the operation. These proteins were involved in many pathological processes, such as neuroinflammatory responses, mitochondrial oxidative stress, impaired synaptic plasticity, and neuronal cell apoptosis. Also, the dysregulated proteins were involved in MAPK, AMPK, and ErbB signaling pathways.

CONCLUSION

The occurrence of PND could be attributed to multiple mechanisms.

摘要

目的

比较围手术期神经认知障碍（PND）小鼠和正常对照小鼠海马组织中的差异表达蛋白，探讨 PND 的可能机制。

方法

将小鼠随机分为 PND 组（n=9）和对照组（n=9）。PND 组小鼠在异氟烷麻醉下接受开放性胫骨骨折伴髓内固定治疗，对照组小鼠接受单纯吸氧而不进行手术。采用 Morris 水迷宫实验、旷场实验和恐惧条件反射实验检测两组小鼠的认知功能。采用高效液相色谱-质谱联用（HPLC-MS/MS）分析小鼠海马的蛋白质表达。通过基因本体（GO）和京都基因与基因组百科全书（KEGG）通路富集分析探讨失调蛋白的主要功能。

结果

术后第 1、3、7 天，PND 组与对照组小鼠共有 21 种蛋白表达存在差异。这些蛋白参与了许多病理过程，如神经炎症反应、线粒体氧化应激、突触可塑性受损和神经元细胞凋亡。此外，失调蛋白还参与了 MAPK、AMPK 和 ErbB 信号通路。

结论

PND 的发生可能涉及多种机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/4b4eecafa13a/40001_2021_599_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/eccb12d4487e/40001_2021_599_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/c5a19d734dbd/40001_2021_599_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/26587d428ab7/40001_2021_599_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/3ab4436266d4/40001_2021_599_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/d8e1cd9584c8/40001_2021_599_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/9bf65dfa2684/40001_2021_599_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/0d4c0e0a63ae/40001_2021_599_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/110dd8720f26/40001_2021_599_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/4b4eecafa13a/40001_2021_599_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/eccb12d4487e/40001_2021_599_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/c5a19d734dbd/40001_2021_599_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/26587d428ab7/40001_2021_599_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/3ab4436266d4/40001_2021_599_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/d8e1cd9584c8/40001_2021_599_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/9bf65dfa2684/40001_2021_599_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/0d4c0e0a63ae/40001_2021_599_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/110dd8720f26/40001_2021_599_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/8565051/4b4eecafa13a/40001_2021_599_Fig9_HTML.jpg

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