Hobkirk R, Fletcher J M, Choi H Y
J Steroid Biochem. 1987 Mar;26(3):413-6. doi: 10.1016/0022-4731(87)90109-9.
The pattern of estrone sulfate sulfohydrolase (estrogen sulfatase) development in the brain of rat, mouse and guinea pig has been established by assaying whole homogenates. Activity was measurable in each species from the fetal state to adulthood. Maximum brain content was reached at about 20 days of age in rat, 14 days in mouse and 15 days in guinea pig. A considerable decrease occurred between 14 days and adulthood in mouse and lesser decreases were seen in rat and guinea pig. The subcellular distribution of enzyme in rat and mouse brain appeared to change from the immature to the adult state. No major differences in enzyme activity occurred between the sexes at any age. Tissue concentration of enzyme in the hypothalamic-preoptic area of rat and mouse was similar to that in the remainder of the brain. In guinea pig the brain concentration was slightly lower than that of the hypothalamic-preoptic region. Sulfatase content of the pituitary was low in all 3 species but the tissue concentration was considerably higher than that of brain, particularly in rat and mouse. Apparent Km values for brain sulfatase were in the range 6-17 microM, with no striking sex difference. Apparent Km's for pituitary sulfatase of immature rat and guinea pig were similar to those for brain in the same animals but that for mouse pituitary (0.9 microM) was much lower. It is unlikely that brain or pituitary sulfatase is by itself, a major factor in making available potentially active estrogen for use during differential sex development in these species.
通过对全组织匀浆进行测定，已确定大鼠、小鼠和豚鼠大脑中硫酸雌酮硫酸酯酶（雌激素硫酸酯酶）的发育模式。在每个物种中，从胎儿期到成年期均可检测到该酶的活性。大鼠在约20日龄时脑内含量达到最高，小鼠为14日龄，豚鼠为15日龄。小鼠在14日龄至成年期之间酶活性大幅下降，大鼠和豚鼠的下降幅度较小。大鼠和小鼠大脑中该酶的亚细胞分布似乎从幼年状态转变为成年状态。在任何年龄段，两性之间的酶活性均无显著差异。大鼠和小鼠下丘脑-视前区的酶组织浓度与大脑其他部位相似。在豚鼠中，大脑浓度略低于下丘脑-视前区。在所有这三个物种中，垂体的硫酸酯酶含量都很低，但组织浓度远高于大脑，特别是在大鼠和小鼠中。大脑硫酸酯酶的表观Km值在6-17微摩尔范围内，无明显性别差异。未成熟大鼠和豚鼠垂体硫酸酯酶的表观Km值与同一动物大脑中的相似，但小鼠垂体的表观Km值（0.9微摩尔）要低得多。在这些物种的性别分化发育过程中，大脑或垂体硫酸酯酶本身不太可能是提供潜在活性雌激素以供利用的主要因素。
