Hobkirk R, Cardy C, Nilsen M, Saidi F
J Steroid Biochem. 1982 Jul;17(1):71-6. doi: 10.1016/0022-4731(82)90594-5.
Estrone sulfatase activity is widespread in guinea pig tissues. Whole homogenates of adult testis, uterus, lung, adrenal, amnion, ovary, chorion, small intestine, placenta, spleen, kidney and liver exhibit approximately descending order of specific activity. Certain properties, including pH requirement, lack of inhibition by inorganic sulfate and magnitude of estimated Km values, are similar to that for arylsulfatase C of rat liver. Of the subcellular fractions prepared from guinea pig tissues, microsomes exhibit the highest specific activity although considerable enzyme activity remains associated with large cellular fragments sedimenting at 750 g. The sulfatase activity is readily inhibited by inorganic phosphate even when substrate concentration satisfied zero order kinetics. Rat liver arylsulfatase C is not inhibited under these conditions. Sensitivity of the guinea pig enzyme activity to inhibition by a variety of steroids and related compounds, is markedly less than for rat liver. Diethylstilbestrol(DES) strongly inhibits the rat liver enzyme but has little effect on the guinea pig liver system. Guinea pig testicular activity is suppressed to a degree intermediate between these extremes by increasing DES concentration. In guinea pig lung, kidney, and possibly liver, elevated fetal enzyme activities decrease from neonatal to adult life. Testicular activity appears to follow the opposite trend. Uterine enzyme activity is not markedly affected by pregnancy.
硫酸雌酮酶活性在豚鼠组织中广泛存在。成年睾丸、子宫、肺、肾上腺、羊膜、卵巢、绒毛膜、小肠、胎盘、脾脏、肾脏和肝脏的全匀浆呈现出特定活性大致递减的顺序。某些特性，包括pH要求、不受无机硫酸盐抑制以及估计的Km值大小，与大鼠肝脏的芳基硫酸酯酶C相似。在从豚鼠组织制备的亚细胞组分中，微粒体呈现出最高的比活性，尽管相当一部分酶活性仍与以750g沉降的大细胞碎片相关。即使底物浓度满足零级动力学，硫酸酯酶活性也很容易被无机磷酸盐抑制。在这些条件下，大鼠肝脏芳基硫酸酯酶C不受抑制。豚鼠酶活性对多种类固醇及相关化合物抑制作用的敏感性明显低于大鼠肝脏。己烯雌酚（DES）强烈抑制大鼠肝脏的酶，但对豚鼠肝脏系统影响很小。随着DES浓度增加，豚鼠睾丸活性受到的抑制程度介于这两个极端情况之间。在豚鼠的肺、肾脏以及可能的肝脏中，胎儿期升高的酶活性从新生儿期到成年期逐渐降低。睾丸活性似乎呈现相反的趋势。子宫酶活性不受妊娠的明显影响。
