Koochaki Mahsa, Hendi Amirreza, Ghasemi Mahmood, Seyedjafari Ehsan, Hamidain Mehdi, Chiniforush Nasim
Department of Oral and Maxillofacial Disease, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Dental Sciences Research Center, Department of Prosthodontics, School of Dentistry, Guilan University of Medical Sciences, Rasht, Iran.
J Lasers Med Sci. 2021 Aug 30;12:e47. doi: 10.34172/jlms.2021.47. eCollection 2021.
This study aimed to compare the effects of root biomodification by citric acid and antimicrobial photodynamic therapy (aPDT) with LED and laser on the proliferation of human gingival fibroblasts (HGFs). This in vitro experimental study evaluated 60 single-rooted teeth extracted due to periodontal disease. The teeth underwent scaling and root planing (SRP), and then 5 × 5 mm blocks were prepared from the cervical area of the teeth 1 mm apical to the cementoenamel junction. The blocks were divided into 4 groups (n=15 blocks): SRP alone (control), SRP + citric acid, SRP + toluidine blue (TBO) + LED light, and SRP + TBO + laser. HGFs were seeded on the surface of the samples, and the methyl thiazolyl tetrazolium (MTT) assay was performed after 24, 48 and 72 hours. Group comparisons were performed using repeated measures ANOVA, while pairwise comparisons of the time points were performed by an LSD test. Cell proliferation was higher in all experimental groups at 48 and 72 hours, compared with 24 hours ( < 0.05). Cell proliferation was significantly different in the citric acid group at 24 hours ( = 0.016) and 48 hours ( = 0.015), compared with other groups. However, cell proliferation was not significantly different in the aPDT group with LED Photosan and a diode laser at 24 and 48 hours ( > 0.05). aPDT and citric acid can enhance the proliferation of HGFs on dentin blocks. Further studies can pave the way for their future use in the clinical setting.
本研究旨在比较柠檬酸和采用发光二极管（LED）及激光的抗菌光动力疗法（aPDT）对人牙龈成纤维细胞（HGFs）增殖的牙根生物改性效果。这项体外实验研究评估了60颗因牙周病拔除的单根牙。这些牙齿接受了龈下刮治和根面平整（SRP），然后从牙颈部区域、牙骨质釉质界根尖1mm处制备5×5mm的块状物。将这些块状物分为4组（每组n = 15个块状物）：单纯SRP（对照组）、SRP + 柠檬酸、SRP + 甲苯胺蓝（TBO） + LED光，以及SRP + TBO + 激光。将HGFs接种在样本表面，并在24、48和72小时后进行甲基噻唑基四氮唑（MTT）检测。使用重复测量方差分析进行组间比较，而时间点的两两比较则通过最小显著差异（LSD）检验进行。与24小时相比，所有实验组在48和72小时时细胞增殖更高（P < 0.05）。与其他组相比，柠檬酸组在24小时（P = 0.016）和48小时（P = 0.015）时细胞增殖有显著差异。然而，使用LED光的Photosan和二极管激光的aPDT组在24和48小时时细胞增殖无显著差异（P > 0.05）。aPDT和柠檬酸可增强牙本质块上HGFs的增殖。进一步的研究可为它们未来在临床环境中的应用铺平道路。
