College of Animal Science, Guizhou University, Guiyang, China.
Sichuan Academy of Grassland Science, Chengdu, China.
Anim Sci J. 2021 Jan-Dec;92(1):e13656. doi: 10.1111/asj.13656.
Two experiments were conducted to investigate the bacterial community of fresh and ensiled paper mulberry prepared with or without lactic acid bacteria (LAB) inoculants in South China. In Experiment 1, the bacterial community, chemical composition, and fermentation products of paper mulberry were analyzed. The results showed that fresh paper mulberry had high crude protein content, buffering capacity value, and amounts of uncultured bacteria. Ensiled paper mulberry showed poor fermentation with high pH value, ammonia-N content, and butyric acid content. In addition, Enterobacter was the dominant genus in silage, followed by Lactobacillus and Enterococcus. Water-soluble carbohydrates, ammonia-N, propionic acid, pH, and lactic acid (LA) were the main factors affecting bacterial community of silage. In Experiment 2, the BP17 (Lactobacillus plantarum) from natural fermented paper mulberry silage and two commercial inoculants (Silage-help [SH] and Chikuso-1 [CH]) were used as additives. Compared with other treatments, BP17 inoculant decreased (p < 0.05) pH and ammonia-N content and increased (p < 0.05) LA content of silage. Inoculation of BP17 also increased the dominance of desirable Lactobacillus and inhibited the growth of harmful bacteria in silage. These results confirmed that paper mulberry could be ensiled and epiphytic LAB inoculant can improve its fermentation quality.
两项实验在中国南方进行，以研究新鲜和青贮的桑树叶的细菌群落，其中一些添加了或未添加乳酸菌（LAB）接种物。在实验 1 中，分析了桑树叶的细菌群落、化学成分和发酵产物。结果表明，新鲜桑树叶粗蛋白含量、缓冲能力值和未培养细菌数量高。青贮桑树叶发酵不良，pH 值、氨态氮含量和丁酸含量高。此外，肠杆菌属是青贮饲料中的优势属，其次是乳酸杆菌属和肠球菌属。水溶性碳水化合物、氨态氮、丙酸、pH 值和乳酸（LA）是影响青贮饲料细菌群落的主要因素。在实验 2 中，将天然发酵的桑树叶青贮饲料中的 BP17（植物乳杆菌）和两种商业接种剂（Silage-help [SH]和 Chikuso-1 [CH]）用作添加剂。与其他处理相比，BP17 接种剂降低（p < 0.05）了青贮饲料的 pH 值和氨态氮含量，增加（p < 0.05）了 LA 含量。BP17 的接种还增加了理想的乳杆菌的优势，并抑制了青贮饲料中有害细菌的生长。这些结果证实了桑树叶可以青贮，并且可以通过添加附着的 LAB 接种剂来改善其发酵质量。
