Cheng Qiming, Li Maoya, Fan Xueying, Chen Yulian, Sun Hong, Xie Yixiao, Zheng Yulong, Chen Chao, Li Ping
College of Animal Science, Guizhou University, Guiyang, China.
Sichuan Academy of Grassland Sciences, Chengdu, China.
Front Microbiol. 2022 Aug 25;13:973500. doi: 10.3389/fmicb.2022.973500. eCollection 2022.
This study aimed to isolate, characterize, and identify lactic acid bacteria (LAB) strains from various sources and evaluate their effects on the nutritional quality, fermentation characteristics, and microbial compositions of paper mulberry (PM) after 60 days of ensiling. Forty-nine LAB strains were isolated from silage, pickle, and fresh PM leaves; three of these strains (, YC1; , PC3; and , BP17) and one commercial inoculant Gaofuji (GFJ) were subsequently used. Compared with other treatments, PC3 and BP17 increased ( < 0.05) the LAB count and crude protein content and decreased ( < 0.05) the molds and coliform bacteria counts, pH, and ammonia-N content of PM silages. BP17 and PC3 increased the relative abundance and decreased that of and , improving PM silage quality. Therefore, PC3 and BP17 can improve the fermentation quality of PM silage and could be used as silage starter cultures.
本研究旨在从不同来源分离、表征和鉴定乳酸菌(LAB)菌株,并评估其在青贮60天后对构树(PM)营养品质、发酵特性和微生物组成的影响。从青贮饲料、泡菜和新鲜构树叶中分离出49株乳酸菌菌株;随后使用其中3株菌株(,YC1;,PC3;和,BP17)以及一种商业接种剂高富基(GFJ)。与其他处理相比,PC3和BP17增加了(<0.05)构树青贮饲料中的乳酸菌数量和粗蛋白含量,并降低了(<0.05)霉菌和大肠菌群数量、pH值和氨氮含量。BP17和PC3增加了的相对丰度,降低了和的相对丰度,提高了构树青贮饲料的质量。因此,PC3和BP17可以改善构树青贮饲料的发酵品质,可作为青贮发酵剂使用。