Vernadsky Institute of Geochemistry and Analytical Chemistry, 119991 Moscow, Russian Federation.
Institute of Biochemistry and Physiology of Plants and Microorganisms, 410049 Saratov, Russian Federation.
Anal Chem. 2021 Nov 16;93(45):14918-14922. doi: 10.1021/acs.analchem.1c04236. Epub 2021 Nov 4.
Metalloproteins have many different functions such as storage and transport of proteins, enzymes, signal transduction proteins, etc. Herein, for a selection of gold nanoparticles differing in shape, size, charge, and surface modification, the binding behavior in human serum was assessed with respect to metal-containing proteins. Our results based on sector-field ICP-MS measurements and a simple calculation algorithm indicate the possible involvement of proteins, incorporating Cu and Fe, in the formation of the biomolecular layer around the particle surface. Given that such binding encompasses a substantial amount of copper and iron within the serum proteome (>50%) at a calculated nanoparticle dose, it may result in depleting their biological functions and should be taken into account when selecting lead candidates with an improved biocompatibility.
金属蛋白具有许多不同的功能，如蛋白质、酶、信号转导蛋白等的储存和运输。在此，针对不同形状、大小、电荷和表面修饰的金纳米粒子的选择，评估了它们在人血清中与含金属蛋白的结合行为。我们基于扇形区 ICP-MS 测量和简单计算算法的结果表明，蛋白质（包括 Cu 和 Fe）可能参与了形成粒子表面生物分子层的过程。鉴于在计算的纳米颗粒剂量下，血清蛋白质组中这种结合包含大量的铜和铁（>50%），这可能导致它们的生物功能丧失，在选择具有改善生物相容性的候选物时应考虑这一点。
