Vernadsky Institute of Geochemistry and Analytical Chemistry, 119991, Moscow, Russian Federation.
Chair of Analytical Chemistry, Warsaw University of Technology, 00664, Warsaw, Poland.
Talanta. 2021 Jul 1;229:122287. doi: 10.1016/j.talanta.2021.122287. Epub 2021 Mar 11.
A great variety of magnetic nanomaterials are entering preclinical investigations with the objective to select the most promising candidates for diagnostic and therapeutic applications. For an analytical approach to be used as a high-throughput screening tool, simple and cost-efficient sample preparation protocol is a basiс prerequisite. Here, we demonstrate how the application of continuous magnetic field allows for quantitatively separating iron oxide magnetic nanoparticles from a mixture with human serum to facilitate monitoring of their biomolecular interactions with high-resolution inductively coupled plasma mass spectrometry. By measuring the signals of sulfur and metal isotopes, it is possible to monitor the formation of the protein corona and alterations in the concentrations of relevant metals due to binding of specific metalloproteins, respectively.
大量的磁性纳米材料正在进入临床前研究，目的是选择最有前途的候选物用于诊断和治疗应用。为了使分析方法能够作为高通量筛选工具，简单且经济高效的样品制备方案是基本前提。在这里，我们展示了如何应用连续磁场从混合物中定量分离氧化铁磁性纳米颗粒，以方便使用高分辨率电感耦合等离子体质谱法监测它们与生物分子的相互作用。通过测量硫和金属同位素的信号，可以分别监测蛋白质外壳的形成以及由于特定金属蛋白酶结合导致相关金属浓度的变化。
