College of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao, People's Republic of China.
Anal Methods. 2021 Nov 18;13(44):5377-5382. doi: 10.1039/d1ay01500a.
It is necessary to develop nanoperoxidases with high activity to construct a fast and cheap sensing platform for real-time detection of some pollutants. In this study, the as-prepared N and S co-doped core-shell cobaltosic oxide nanospheres (N,S-CoO) exhibit excellent peroxidase-like activity. The oxidation reaction of the colorless chromogenic substrate TMB by HO is used to evaluate the peroxidase-like behaviors of N,S-CoO. As expected, the N,S-CoO nanospheres accelerated the oxidation of TMB accompanied by a blue shift only in 1 min. Thus, the N,S-CoO nanoperoxidase exhibits high affinity towards TMB ( = 0.072 mM) and HO ( = 3.78 mM). Moreover, as the catalytic process of N,S-CoO can be inhibited in the presence of catechol, a fast inexpensive colorimetric sensor of catechol with high sensitivity and good selectivity was constructed. The enhanced catalytic activity of N,S-CoO is attributed to some active species, including h and ˙O, owing to the more active sites on N,S-CoO. The colorimetric method has been validated by detecting catechol in real water samples for practical application.
为了构建用于实时检测一些污染物的快速且廉价的传感平台，有必要开发具有高活性的纳米过氧化物酶。在这项研究中，所制备的 N 和 S 共掺杂核壳结构的钴酸纳米球（N，S-CoO）表现出优异的过氧化物酶样活性。无色显色底物 TMB 与 HO 的氧化反应用于评估 N，S-CoO 的过氧化物酶样行为。如预期的那样，N，S-CoO 纳米球在 1 分钟内仅加速 TMB 的氧化，伴随蓝移。因此，N，S-CoO 纳米过氧化物酶对 TMB（=0.072mM）和 HO（=3.78mM）具有高亲和力。此外，由于儿茶酚的存在会抑制 N，S-CoO 的催化过程，因此构建了一种具有高灵敏度和良好选择性的儿茶酚快速廉价的比色传感器。N，S-CoO 的增强的催化活性归因于一些活性物质，包括 h 和 ˙O，这是由于 N，S-CoO 上具有更多的活性位点。该比色法已通过检测实际水样中的儿茶酚来验证，可用于实际应用。
