College of Veterinary Medicine, China Agricultural University, No.2 Yuanmingyuan West Road, Beijing, 100193, China.
College of Veterinary Medicine, China Agricultural University, No.2 Yuanmingyuan West Road, Beijing, 100193, China; Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Preventive Medical Research, Beijing, 100013, China.
Talanta. 2022 Jan 15;237:122919. doi: 10.1016/j.talanta.2021.122919. Epub 2021 Oct 4.
Diazepam (DZP) was routinely prescribed to a large population troubled with anxiety disorders. However, due to the overuse and misuse, DZP and its chlorination disinfection byproduct 2-methylamino-5-chlorobenzophenone (MACB) caused environmental pollution and can be detected ubiquitously in drinking water in Beijing, China. However, little information is known about the metabolic dynamics of MACB. Here, we established desorption electrospray ionization mass spectrometry (DESI-MS) imaging method to visually and quantitatively assess the distribution and metabolism of MACB in zebrafish. The results showed that MACB specifically accumulated in spinal cord particularly in female zebrafish. Meanwhile, the accumulation of MACB could pass through the blood-brain barrier (BBB) and induced microglial phagocytosis of neurons. Therefore, the intervention strategies should be explored to restrict the release of such substances, eliminating the potential risks for both human beings and the eco-environment.
地西泮（DZP）被常规用于治疗患有焦虑症的大量人群。然而，由于过度使用和滥用，DZP 及其氯化消毒副产物 2-甲基氨基-5-氯二苯甲酮（MACB）造成了环境污染，并且在中国北京的饮用水中普遍存在。然而，关于 MACB 的代谢动力学知之甚少。在这里，我们建立了解吸电喷雾电离质谱成像（DESI-MS）方法，以直观和定量评估 MACB 在斑马鱼中的分布和代谢。结果表明，MACB 特异性地积累在脊髓中，特别是在雌性斑马鱼中。同时，MACB 的积累可以穿透血脑屏障（BBB）并诱导小胶质细胞吞噬神经元。因此，应该探索干预策略来限制此类物质的释放，消除对人类和生态环境的潜在风险。
