State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China; School of Pharmacy, Jiangsu Ocean University, Lianyungang, 222005, China.
Talanta. 2022 Jan 15;237:122958. doi: 10.1016/j.talanta.2021.122958. Epub 2021 Oct 9.
Immunoglobulin G (IgG) is a significant ingredient of immunological activity in milk and colostrum, the activity and content of which is easily disturbed by potentially conditional variant during sterilization. Therefore, developing robust methods for the detection of IgG levels in milk is especially important. Herein, protein A from the Staphylococcus aureus functionalized silica colloidal crystalline film (SCC@SPA) sensing unit combined with ordered porous layer interferometry (OPLI) for IgG detection in untreated bovine milk was developed. Calibration curves in milk and buffer were set up by the variations of the optical thickness (OT) of the sensing unit after the IgG association and dissociation phases. The influence of temperature on the level of IgG was evaluated. Furthermore, the identification of IgG levels with pasteurized milk and ultrahigh temperature (UHT) sterilized milk from the market randomly was successfully carried out without any sample pretreatment. More importantly, compared with other methods, this novel method has the advantages of convenient operation, low cost, and suitability for point-of-care (POC) testing.
免疫球蛋白 G（IgG）是乳和初乳中免疫活性的重要成分，其活性和含量很容易在灭菌过程中受到潜在条件变体的干扰。因此，开发用于检测乳中 IgG 水平的稳健方法尤为重要。在此，我们开发了一种基于金黄色葡萄球菌蛋白 A 功能化的硅胶体晶体膜（SCC@SPA）传感单元与有序多孔层干涉（OPLI）相结合的方法，用于检测未经处理的牛乳中的 IgG。通过 IgG 结合和解离阶段后传感单元的光学厚度（OT）变化，在牛奶和缓冲液中建立了校准曲线。评估了温度对 IgG 水平的影响。此外，无需任何样品预处理，成功地对市售巴氏杀菌乳和超高温（UHT）灭菌乳中的 IgG 水平进行了识别。更重要的是，与其他方法相比，这种新方法具有操作简便、成本低、适用于即时检测（POC）的优点。
