Department of Chemical Science and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133, Rome, Italy.
Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, 119121, Moscow, Russia.
Talanta. 2022 Jan 15;237:122869. doi: 10.1016/j.talanta.2021.122869. Epub 2021 Sep 11.
Point-of-care devices have attracted a huge interest by the scientific community because of the valuable potentiality for rapid diagnosis and precision medicine through cost-effective and easy-to-use devices for on-site measurement by unskilled personnel. Herein, we reported a smartphone-assisted electrochemical device consisted of a screen-printed electrode modified with carbon black nanomaterial and a commercially available smartphone potentiostat i.e. EmStat3 Blue, for sensitive detection of tyrosine. Once optimized the conditions, tyrosine was detected in standard solutions by square wave voltammetry, achieving a linear range comprised between 30 and 500 μM, with a detection limit equal to 4.4 μM. To detect tyrosine in serum, the interference of another amino acid i.e. tryptophan was hindered using a sample treatment with an extraction cartridge. The agreement of results analyzing serum samples with HPLC reference method and with the developed smart sensing system demonstrated the suitability of this smartphone-assisted sensing tool for cost-effective and rapid analyses of tyrosine in serum samples.
由于能够通过经济高效且易于使用的现场测量设备实现快速诊断和精准医疗，具有巨大的潜力，因此，即时检测设备吸引了科学界的极大兴趣。在此，我们报道了一种智能手机辅助电化学设备，它由一个经过碳纳米材料修饰的丝网印刷电极和一个市售的智能手机电化学工作站 EmStat3 Blue 组成，用于灵敏检测酪氨酸。优化条件后，通过方波伏安法在标准溶液中检测到酪氨酸，线性范围为 30-500 μM，检测限为 4.4 μM。为了检测血清中的酪氨酸，使用提取试剂盒对样品进行处理，以阻止另一种氨基酸色氨酸的干扰。通过与 HPLC 参考方法和开发的智能传感系统分析血清样品的结果进行比较，表明这种智能手机辅助传感工具非常适合用于经济高效且快速分析血清样品中的酪氨酸。
