Department of Chemical Science and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, Rome, 00133, Italy.
Laboratory of Clinical Pharmacology, "Franco Ghezzo", Department of Clinical and Biological Sciences, University of Turin, S. Luigi Gonzaga Hospital, 10043, Orbassano, TO, Italy.
Anal Bioanal Chem. 2023 Mar;415(6):1149-1157. doi: 10.1007/s00216-023-04537-6. Epub 2023 Jan 26.
The fast-growing healthcare demand for user-friendly and affordable analytical tools is driving the efforts to develop reliable platforms for the customization of therapy based on individual health conditions. In this overall scenario, we developed a paper-based electrochemical sensor for the quantification of iron ions in serum as a cost-effective sensing tool for the correct supplement administration. In detail, the working electrode of the screen-printed device has been modified with a nanocomposite constituted of carbon black and gold nanoparticles with a drop-casting procedure. Square wave voltammetry has been adopted as an electrochemical technique. This sensor was further modified with Nafion for iron quantification in serum after sample treatment with trifluoroacetic acid. Under optimized conditions, iron ions have been detected with a LOD down to 0.05 mg/L and a linearity up to 10 mg/L in standard solution. The obtained results have been compared with reference methods namely commercial colorimetric assay and atomic absorption spectroscopy, obtaining a good correlation within the experimental errors. These results demonstrated the suitability of the developed paper-based sensor for future applications in precision medicine of iron-deficiency diseases.
快速增长的医疗保健需求对用户友好且经济实惠的分析工具的推动，促使人们努力开发可靠的平台，根据个体健康状况定制治疗方案。在这种总体情况下，我们开发了一种基于纸张的电化学传感器，用于定量检测血清中的铁离子，作为一种用于正确补充剂给药的具有成本效益的传感工具。具体来说，通过滴涂程序，用包含碳黑和金纳米粒子的纳米复合材料对丝网印刷器件的工作电极进行了修饰。采用方波伏安法作为电化学技术。在优化条件下，在经过三氟乙酸处理的血清样本中，该传感器在 0.05mg/L 的检测限下对铁离子进行了检测，线性范围可达 10mg/L。将得到的结果与参考方法（即商业比色法和原子吸收光谱法）进行了比较，在实验误差范围内得到了很好的相关性。这些结果表明，开发的基于纸张的传感器适用于未来在缺铁性疾病的精准医疗中的应用。
