Du Renpeng, Ping Wenxiang, Song Gang, Ge Jingping
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500, PR China; Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080, PR China.
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500, PR China; Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080, PR China.
Int J Biol Macromol. 2021 Dec 15;193(Pt A):693-701. doi: 10.1016/j.ijbiomac.2021.10.176. Epub 2021 Oct 30.
A new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber. The structure and antibacterial properties of the nanofibers were evaluated. Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I. X-ray photoelectron spectrometer (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM) revealed that the bacteriocin and BC were successfully compounded, and the structure of BC-B nanofiber was tighter than BC nanofiber, with lower porosity, swelling ratio and water vapor transmission rate (WVTR). The tensile strength and Young's modulus of BC-B nanofibers were 13.28 ± 1.26 MPa and 132.10 ± 4.92 MPa, respectively, higher than that of BC nanofiber (6.12 ± 0.87 MPa and 101.59 ± 5.87 MPa), indicating that bacteriocin enhance the mechanical properties of BC nanofiber. Furthermore, the BC-B nanofibers exhibited significant thermal stability, antioxidant capacity and antibacterial activity than BC nanofiber. Therefore, bacteriocin-loaded BC nanofiber may be used as antimicrobial agents in active food packaging and medical material.
分离并鉴定出一种新的产细菌纤维素（BC）的玫瑰色葡萄糖杆菌HDX-1，并采用一种简单、低成本的复合方法生物合成副干酪乳杆菌1∙7细菌素BC（BC-B）纳米纤维。对纳米纤维的结构和抗菌性能进行了评估。固态核磁共振（NMR）、傅里叶变换红外光谱（FT-IR）和X射线衍射（XRD）分析表明，BC和BC-B纳米纤维具有纤维素I的典型晶体形式。X射线光电子能谱仪（XPS）、扫描电子显微镜（SEM）和原子力显微镜（AFM）显示，细菌素与BC成功复合，且BC-B纳米纤维的结构比BC纳米纤维更紧密，孔隙率、溶胀率和水蒸气透过率（WVTR）更低。BC-B纳米纤维的拉伸强度和杨氏模量分别为13.28±1.26 MPa和132.10±4.92 MPa，高于BC纳米纤维（6.12±0.87 MPa和101.59±5.87 MPa），表明细菌素增强了BC纳米纤维的力学性能。此外，BC-B纳米纤维比BC纳米纤维表现出显著的热稳定性、抗氧化能力和抗菌活性。因此，载有细菌素的BC纳米纤维可作为活性食品包装和医用材料中的抗菌剂。
