Key Laboratory of Environmental and Applied Microbiology, CAS, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
Center for Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, Ghent 9000, Belgium.
Bioresour Technol. 2022 Jan;344(Pt B):126243. doi: 10.1016/j.biortech.2021.126243. Epub 2021 Oct 28.
The mechanisms underlying the advanced performance in anaerobic co-digestion (AcoD) are crucial but remain elusive. This study conducted AcoD of swine manure, rice straw and apple waste (AW, mainly consisting of labile carbon) or fructose (a pure labile carbon), and monitored microbial community abundances, activities and transcriptional profiles in the digestate and on straw. The transformation efficiencies of manure (not straw) to CH were promoted in AcoD co-fed manure and AW (by 39 ± 13%) or fructose (by 65 ± 14%), compared to the control mono-fed manure, implying labile carbon could trigger a priming effect underlying AcoD advantage. Although digestate-associated and straw-associated communities existed in a same bioreactor, the priming effect mainly linked to the former and was attributed to enhancements in deterministic turnover of active communities, in activities of Firmicutes taxa involved in substrate hydrolysis, and in acetoclastic methanogenesis. These findings provide novel insights to elaborate AcoD processes.
在厌氧共消化（AcoD）中，先进性能背后的机制至关重要，但仍难以捉摸。本研究对猪粪、稻草和苹果废物（AW，主要含有易分解碳）或果糖（纯易分解碳）进行了 AcoD 处理，并监测了消化物和稻草上微生物群落丰度、活性和转录谱的变化。与对照单喂粪肥相比，共喂粪肥和 AW（提高 39±13%）或果糖（提高 65±14%）促进了粪肥（非稻草）向 CH 的转化效率，这表明易分解碳可能引发了 AcoD 优势的激发效应。尽管同一个生物反应器中存在与消化物相关和与稻草相关的群落，但激发效应主要与前者有关，归因于活性群落的确定性周转增强、参与底物水解的厚壁菌门分类群的活性增强，以及乙酸营养型产甲烷作用增强。这些发现为阐述 AcoD 过程提供了新的见解。
