Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China.
Hangzhou Energy Environmental Engineering Ltd, Hangzhou 310020, China.
Bioresour Technol. 2022 Jul;355:127262. doi: 10.1016/j.biortech.2022.127262. Epub 2022 May 5.
Layered inoculation can achieve rapid start-up and promote methanation performance of anaerobic digesters. Daily specific methane yield (SMY) rapidly increased to 2.93 mL/g VS/d during 0-13 days, and cumulative SMY reached 212 mL/g VS in the solid-state anaerobic co-digestion (SS-AcoD) of pig manure and corn straw. Data were collected at macro-, micro-, and genetic-levels of each substrate layer. The results showed that layered inoculation could improve volatile fatty acids utilization and prevent adverse effects of high total ammonium nitrogen concentrations. Layered inoculation accelerated hydrolysis, acidification, and methanogenesis of substrates, as evidenced by the efficient inoculation of Bacteroidetes, Anaerolineales, Methanosphaerula, and Methanothrix, which were primarily from inocula. The various stages of SS-AcoD were synergistically initiated during the first 13 days, and acetoclastic pathway was boosted. These results further explain why layered inoculation is an efficient method for improving methanation performance of SS-AcoD and achieving efficient utilization of organic solid waste.
分层接种可以实现快速启动，并促进厌氧消化器的甲烷化性能。在 0-13 天内，每日特定甲烷产量（SMY）迅速增加到 2.93 毫升/克 VS/天，固态厌氧共消化（SS-AcoD）中猪粪和玉米秸秆的累积 SMY 达到 212 毫升/克 VS。在每个底物层的宏观、微观和遗传水平上都收集了数据。结果表明，分层接种可以提高挥发性脂肪酸的利用率，并防止高总铵氮浓度的不利影响。分层接种加速了底物的水解、酸化和甲烷生成，这可以从接种物中主要存在的拟杆菌门、厌氧杆菌目、产甲烷菌属和甲烷丝菌属的有效接种得到证明。在最初的 13 天内，SS-AcoD 的各个阶段协同启动，并且促进了乙酰辅酶 A 途径。这些结果进一步解释了为什么分层接种是提高 SS-AcoD 甲烷化性能和实现有机固体废物高效利用的有效方法。
