Pan Qiqun, Li Tangfei, Luo Zhangqin, Pan Pengji
Department of Hematology, Zhuji Affiliated Hospital of Shaoxing University, Zhuji, Zhejiang 311800, China.
Department of Hematology, Yongchuan Hospital of Chongqing Medical University, Chongqing 402160, China.
Evid Based Complement Alternat Med. 2021 Oct 26;2021:6101060. doi: 10.1155/2021/6101060. eCollection 2021.
The multiple myeloma is a malignant clonal tumor of bone marrow plasma cells that is incurable and inevitably recurrent. The mechanisms of progression include tumor cell metastasis, immune escape, resistance to apoptosis, and malignant proliferation. The cysteine-rich secreted acidic protein is closely related to the growth, development, remodeling, and repair of cells and tissues. In our study, we divided myeloma patients and patients with other blood diseases into groups and measured the cysteine-rich secreted acidic protein (SPARC) content in the serum of different groups of patients as well as the prognostic differences. The U266 cells were transfected with interfering vectors and overexpressed SPARC vectors to determine the physiological functions of MM cells. Our results showed that SPARC was highly expressed in MM and the survival rate of the high SPARC expression group was lower than that of the low expression group. Interfering SPARC vectors inhibited cancer cell proliferation, migration, and invasion and promoted apoptosis. Overexpression of SPARC vectors promoted cancer cell development. SPARC affected the patient's disease development by regulating the biological behavior of the MM cells.
多发性骨髓瘤是一种骨髓浆细胞的恶性克隆性肿瘤，无法治愈且不可避免会复发。其进展机制包括肿瘤细胞转移、免疫逃逸、抗凋亡及恶性增殖。富含半胱氨酸的分泌性酸性蛋白与细胞和组织的生长、发育、重塑及修复密切相关。在我们的研究中，我们将骨髓瘤患者和其他血液病患者分组，并测量不同组患者血清中富含半胱氨酸的分泌性酸性蛋白（SPARC）含量以及预后差异。用干扰载体和过表达SPARC载体转染U266细胞，以确定骨髓瘤细胞的生理功能。我们的结果显示，SPARC在骨髓瘤中高表达，SPARC高表达组的生存率低于低表达组。干扰SPARC载体可抑制癌细胞增殖、迁移和侵袭，并促进凋亡。过表达SPARC载体可促进癌细胞发展。SPARC通过调节骨髓瘤细胞的生物学行为影响患者疾病进展。
