Hu Hai-Su, Zhang De-Quan
College of Pharmacy, Dali University Dali 671000, China.
College of Pharmacy, Dali University Dali 671000, China Institute of Materia Medica, Dali University Dali 671000, China.
Zhongguo Zhong Yao Za Zhi. 2021 Oct;46(20):5260-5269. doi: 10.19540/j.cnki.cjcmm.20210720.103.
Gentiana is an important but complicated group in Gentianaceae. The genus covers numerous medicinal plants which are difficult to be identified. In the present study, several medicinal species in Gentiana from Yunnan province, including G. rigescens, G.rhodantha, and G. delavayi, were sequenced using the Illumina HiSeq 2500 system. Three complete chloroplast genome sequences were obtained after assembly and annotation. According to several published genome sequences of G. crassicaulis, the DNA super-barcoding of species in Gentiana was preliminarily carried out. The results revealed that chloroplast genomes of the three species were conservative with short lengths(146 944, 148 992, and 148 796 bp, respectively). The genomes encoded 114 genes, including 78 protein-coding genes, 30 tRNA genes, 4 rRNA genes, and 2 pseudogenes. Furthermore, these medicinal species in Yunnan province were identified using DNA super-barcoding based on chloroplast genomes. The results showed that the Gentiana species could be gathered into monophyletic branches with a high support value(100%). It indicated that DNA super-barcoding possessed obvious advantages in discriminating species in complicated genera. This study is expected to provide a scientific basis for the identification, utilization, and conservation of Gentiana species.
龙胆属是龙胆科中一个重要但复杂的类群。该属包含众多难以鉴定的药用植物。在本研究中，利用Illumina HiSeq 2500系统对云南省的几种龙胆属药用植物进行了测序，包括坚龙胆、红龙胆和滇龙胆。经过组装和注释后获得了三个完整的叶绿体基因组序列。根据已发表的粗茎秦艽的几个基因组序列，初步开展了龙胆属物种的DNA超级条形码研究。结果显示，这三个物种的叶绿体基因组较为保守，长度较短（分别为146 944、148 992和148 796 bp）。这些基因组编码114个基因，包括78个蛋白质编码基因、30个tRNA基因、4个rRNA基因和2个假基因。此外，基于叶绿体基因组利用DNA超级条形码对云南省的这些药用物种进行了鉴定。结果表明，龙胆属物种能够聚为单系分支，支持率很高（100%）。这表明DNA超级条形码在鉴别复杂类属中的物种方面具有明显优势。本研究有望为龙胆属物种的鉴定、利用和保护提供科学依据。
