Huang Cai-Xia, Liu Mi-Li, Zhang Heng-Jia, Chang Lei, Wang Yu-Cai, Yan Ji-Xuan
College of Water Conservancy and Hydropower Engineering, Gansu Agricultural University, Lanzhou, China.
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Shaanxi, China.
Mitochondrial DNA B Resour. 2019 Jul 16;4(2):2596-2597. doi: 10.1080/23802359.2019.1641442.
N. E. Brown (Gentianaceae) is a perennial herb plant and only grows in Qinba Mountains in China. Here, we first characterized the complete nucleotide sequence of chloroplast (cp) genome of via Illumina next generation sequencing platform. The complete chloroplast genome of . was 144,274 bp in length, comprising of a large single copy (LSC) region of 77,353 bp, a small single copy (SSC) region of 17,009 bp, and two inverted repeat regions (IRs) of 24,956 bp. The cp genome contains 127 genes, including 82 protein-coding genes, 35 tRNA, eight rRNA genes, and two pseudogenes. Phylogenetic analysis based on 18 cp genome sequences showed that . closely related to congeneric species.
N. E. Brown(龙胆科)是一种多年生草本植物,仅生长在中国的秦巴山区。在此,我们首次通过Illumina下一代测序平台对其叶绿体(cp)基因组的完整核苷酸序列进行了表征。N. E. Brown的完整叶绿体基因组长度为144,274 bp,由一个77,353 bp的大单拷贝(LSC)区域、一个17,009 bp的小单拷贝(SSC)区域和两个24,956 bp的反向重复区域(IRs)组成。该cp基因组包含127个基因,包括82个蛋白质编码基因、35个tRNA、8个rRNA基因和2个假基因。基于18个cp基因组序列的系统发育分析表明,N. E. Brown与同属物种密切相关。