Qiao Ri-Fa, Zhong Min-Yong, Zhang Min, Yang Lin, DU Xi-Qin, Tuo Fu-Lin, Yuan Jin-Bin
Key Laboratory of Modern Preparation of Traditional Chinese Medicine Under Ministry of Education, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China.
Key Laboratory of Modern Preparation of Traditional Chinese Medicine Under Ministry of Education, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China Nanchang Key Laboratory of Quality Control and Safety Evaluation of Traditional Chinese Medicine, Nanchang Institute for Food and Drug Control Nanchang 330004, China.
Zhongguo Zhong Yao Za Zhi. 2021 Oct;46(20):5291-5303. doi: 10.19540/j.cnki.cjcmm.20210525.307.
Aurantii Fructus is a commonly used qi-regulating medicinal herb in China. Both traditional Chinese medicine theory and modern experimental research demonstrate that Aurantii Fructus has dryness effect, the material basis of which remains unclear. In recent years, spectrum-effect relationship has been widely employed in the study of active ingredients in Chinese medicinal herbs, the research ideas and methods of which have been constantly improved. Based on the idea of spectrum-effect study, the ultra-high perfor-mance liquid chromatography-quadrupole-time of flight mass spectrometry(UHPLC-Q-TOF-MS) fingerprints of different fractions of Aurantii Fructus extract were established for the identification of total components. Then, the dryness effects of the fractions on normal mice and gastrointestinal motility disorder(GMD) rats were systematically compared. Finally, principal component analysis(PCA), Pearson bivariate correlation analysis and orthogonal partial least squares analysis(OPLS) were integrated to identify the dryness components of Aurantii Fructusextract. The results showed that narirutin, naringin, naringenin, poncirin, oxypeucedanin, and eriodictyol-7-O-glucoside had significant correlations with and contributed to the expression of AQP2 in kidney, AQP3 in colon, and AQP5 in submandibular gland, which were the main dryness components in Aurantii Fructus.
枳实是我国常用的理气药。中医理论和现代实验研究均表明枳实具有燥性作用，但其物质基础尚不清楚。近年来，谱效关系已广泛应用于中药活性成分的研究，其研究思路和方法不断完善。基于谱效关系研究的思路，建立了枳实提取物不同部位的超高效液相色谱-四极杆-飞行时间质谱（UHPLC-Q-TOF-MS）指纹图谱，用于鉴定其总成分。然后，系统比较了各部位对正常小鼠和胃肠动力障碍（GMD）大鼠的燥性作用。最后，综合主成分分析（PCA）、Pearson双变量相关分析和正交偏最小二乘法分析（OPLS），鉴定枳实提取物的燥性成分。结果表明，橙皮苷、柚皮苷、柚皮素、枳属苷、氧化前胡素和圣草酚-7-O-葡萄糖苷与肾组织中AQP2、结肠组织中AQP3和颌下腺组织中AQP5的表达显著相关，并对其表达有贡献，这些是枳实的主要燥性成分。
