Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, China.
Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, China; Nanchang Key Laboratory of Quality Control and Safety Evaluation of Traditional Chinese Medicine, Nanchang Institute for Food and Drug Control, Nanchang, 330015,China.
J Ethnopharmacol. 2021 Oct 28;279:114366. doi: 10.1016/j.jep.2021.114366. Epub 2021 Jun 25.
Nowadays, gastrointestinal motility disorders (GMD) have reduced the quality of people's daily life worldwide, but there is still a lack of effective western medicine treatment. Fructus aurantii (FA), a representative regulating-qi herbal medicine, has been widely used to treat GMD in China for thousands of years, but it is not clear that which specific components contribute to the efficacy.
The efficacy differences of various fractions of FA on normal mice and GMD rats were compared, so as to find out the main effective fraction of FA, and to screen the main regulating-qi components based on spectrum-effect relationship and multivariate statistical analysis.
The fingerprints of different fractions of FA were established and main compounds were identified with UHPLC-Q-TOF/MS technique. The promoting gastrointestinal motility activities of FA were evaluated by defecation test, gastric emptying and intestinal propulsion test in mice, and further investigated according to the biochemical analysis of 5-HT, SP, MLT, GAS and VIP in GMD rats' plasma. One-way ANOVA was used to find out the difference of efficacy. The active components were screened through spectrum-effect relationship with PCA-X, Pearson bivariate correlation analysis and OPLS analysis.
Ethyl acetate fraction is the main active fraction, and nine compounds are the major regulating-qi components. The developed spectrum-effect analysis can be used for the screening of bioactive components in natural products with high accuracy and reliability.
现今，胃肠动力障碍（GMD）降低了全球人们的生活质量，但仍缺乏有效的西药治疗方法。枳实（FA）作为一种具有代表性的理气中药，数千年来在中国被广泛用于治疗 GMD，但尚不清楚哪些特定成分具有疗效。
本研究的目的是比较 FA 各馏分对正常小鼠和 GMD 大鼠的疗效差异，以找出 FA 的主要有效馏分，并基于谱效关系和多变量统计分析筛选主要的理气成分。
采用 UHPLC-Q-TOF/MS 技术建立不同馏分的指纹图谱并鉴定主要化合物。通过排便试验、胃排空和肠推进试验评估 FA 促进胃肠动力的作用，进一步根据 GMD 大鼠血浆中 5-HT、SP、MLT、GAS 和 VIP 的生化分析进行研究。采用单因素方差分析找出疗效差异。通过 PCA-X、Pearson 二元相关分析和 OPLS 分析进行谱效关系筛选活性成分。
乙酸乙酯馏分是主要的活性馏分，有九个化合物是主要的理气成分。所开发的谱效分析可用于高准确性和可靠性的天然产物生物活性成分的筛选。
