159363Tangshan Gongren Hospital, Tangshan, China.
Technol Cancer Res Treat. 2021 Jan-Dec;20:15330338211043976. doi: 10.1177/15330338211043976.
To explore the function of the miR-18a-5p/CPEB3 axis in regulating the occurrence of hepatocellular carcinoma (HCC). Differentially expressed miRNAs and mRNAs were acquired by bioinformatics analysis. qRT-PCR was used for miR-18a-5p and CPEB3 mRNA expression detection. Cell functional assays were implemented to examine the biological functions of HCC cells. The binding relationship between miR-18a-5p and CPEB3 was verified by a dual luciferase assay. In HCC, miR-18a-5p was remarkably highly expressed, while CPEB3 was markedly lowly expressed. HCC cell progression was facilitated after cells transfecting miR-18a-5p mimic, whereas silencing miR-18a-5p caused the opposite result. Overexpressing CPEB3 could restore promoting effect of miR-18a-5p on the growth of HCC cells. Oncogene miR-18a-5p accelerates malignant phenotype by suppressing CPEB3. MiR-18a-5p/CPEB3 axis in HCC identified in this study provides a new target for HCC treatment.
探讨 miR-18a-5p/CPEB3 轴在调控肝细胞癌（HCC）发生中的作用。通过生物信息学分析获得差异表达的 miRNAs 和 mRNAs。qRT-PCR 用于检测 miR-18a-5p 和 CPEB3 mRNA 的表达。细胞功能测定用于检测 HCC 细胞的生物学功能。通过双荧光素酶报告基因实验验证 miR-18a-5p 与 CPEB3 的结合关系。在 HCC 中，miR-18a-5p 表达明显上调，而 CPEB3 表达明显下调。转染 miR-18a-5p 模拟物后促进 HCC 细胞的进展，而沉默 miR-18a-5p 则产生相反的结果。过表达 CPEB3 可以恢复 miR-18a-5p 对 HCC 细胞生长的促进作用。癌基因 miR-18a-5p 通过抑制 CPEB3 加速恶性表型。本研究鉴定的 HCC 中的 miR-18a-5p/CPEB3 轴为 HCC 治疗提供了新的靶点。
