Gregorio Paulo Henrique Peitl, Mariani Alessandro Wasum, Brito João Marcelo Lopes Toscano, Santos Bruno José Martini, Pêgo-Fernandes Paulo Manuel
Thoracic Surgery Department, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo - São Paulo, São Paulo, Brazil (Drs Gregorio, Mariani, Brito, Santos, and Fernandes).
J Occup Environ Med. 2021 Nov 1;63(11):956-962. doi: 10.1097/JOM.0000000000002284.
To evaluate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spread inside the healthcare setting using environmental sampling and indoor air quality (IAQ) parameters.
Ward/ICU rooms had IAQ parameters monitored in real-time, including volatile organic compounds and particulate matter. Surface and three air samples with different exposure times were collected in each room and tested for SARS-CoV-2 using quantitative Rt-PCR. Environmental sampling and IAQ data were compared to provide information about viral spread.
SARS-CoV-2 RNA was detected in 6/10 rooms and 9/30 air samples, which is proportionally higher than previous studies. Sampling time confirmed to be crucial to viral detection. No correlations between IAQ parameters could be associated with positive/negative samples even when aerosol-generating procedures were performed.
Environmental sampling of SARS-CoV-2 RNA may be used as an indicator of occupational safety. IAQ is also a potential tool but requires further research.
通过环境采样和室内空气质量（IAQ）参数评估严重急性呼吸综合征冠状病毒2（SARS-CoV-2）在医疗机构内的传播情况。
对病房/重症监护病房（ICU）的房间实时监测IAQ参数，包括挥发性有机化合物和颗粒物。在每个房间采集不同暴露时间的表面样本和三个空气样本，并使用定量逆转录聚合酶链反应（Rt-PCR）检测SARS-CoV-2。比较环境采样和IAQ数据以提供有关病毒传播的信息。
在10个房间中的6个和30个空气样本中的9个检测到SARS-CoV-2 RNA，这一比例高于先前的研究。采样时间被证实对病毒检测至关重要。即使在进行气溶胶生成程序时，IAQ参数与阳性/阴性样本之间也没有相关性。
SARS-CoV-2 RNA的环境采样可作为职业安全的指标。IAQ也是一种潜在工具，但需要进一步研究。
