Medicine and Psychiatry Department, Universidad de Cantabria, Av. Cardenal Herrera Oria, s/n, 39011, Santander, Cantabria, Spain.
Departamento de Ingenierías Química y Biomolecular, Universidad de Cantabria, Avda. Los Castros S/N, 39005, Santander, Cantabria, Spain.
Environ Sci Pollut Res Int. 2022 Sep;29(42):62973-62983. doi: 10.1007/s11356-022-20237-7. Epub 2022 Apr 21.
We aimed to determine the presence of SARS-CoV-2 RNA in indoor and outdoor size-segregated aerosol samples (PM, PM). Five outdoor daily samples were collected between November and December 2020 in an urban/industrial area with relatively high PM levels (Maliaño, Santander, Spain) by using a PM impactor (air flowrate of 30 L/min). In a non-hospital indoor sampling surveillance context, 8 samples in classrooms and 6 samples in the central library-Paraninfo of the University of Cantabria (UC) were collected between April and June 2021 by using personal PM samplers (air flowrate of 3 L/min). Lastly, 8 samples in the pediatric nasopharyngeal testing room at Liencres Hospital, 6 samples from different single occupancy rooms of positive patients, and 2 samples in clinical areas of the COVID plant of the University Hospital Marqués de Valdecilla (HUMV) were collected between January and May 2021. N1, N2 genes were used to test the presence of SARS-CoV-2 RNA by RT-qPCR. SARS-CoV-2 positive detection was only obtained from one fine fraction (PM) sample, corresponding to one occupancy room, where a patient with positive PCR and cough was present. Negative results found in other sampling areas such as the pediatric nasopharyngeal testing rooms should be interpreted in terms of air sampling volume limitation and good ventilation.
我们旨在确定 SARS-CoV-2 RNA 在室内和室外大小分级气溶胶样本（PM）中的存在情况。2020 年 11 月至 12 月期间，在西班牙桑坦德市马利亚诺（Maliaño）一个 PM 水平相对较高的城市/工业区，使用 PM 撞击器（气流速率为 30 L/min）采集了 5 个室外日常样本。在非医院室内采样监测环境中，2021 年 4 月至 6 月期间，使用个人 PM 采样器（气流速率为 3 L/min）在坎塔布里亚大学（UC）的教室中采集了 8 个样本，在中央图书馆-帕拉尼翁（Paraninfo）中采集了 6 个样本。最后，在利恩克雷斯医院（Liencres Hospital）的儿科鼻咽测试室中采集了 8 个样本，在阳性患者的不同单人房间中采集了 6 个样本，在玛奎斯德瓦尔德西利亚大学医院（HUMV）的 COVID 工厂的临床区域中采集了 2 个样本。使用 RT-qPCR 测试 N1、N2 基因以检测 SARS-CoV-2 RNA 的存在情况。仅从一个细颗粒（PM）样本中检测到 SARS-CoV-2 阳性，该样本对应一个有阳性 PCR 和咳嗽症状的患者的居住房间。在儿科鼻咽测试室等其他采样区域发现的阴性结果应根据空气采样量限制和良好的通风情况进行解释。
