Department of Biomolecular Sciences, University of Urbino "Carlo Bo".
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Acta Biomed. 2021 Oct 1;92(S6):e2021438. doi: 10.23750/abm.v92iS6.12238.
The world of work has been profoundly affected by the COVID-19 pandemic since workplace activities involving close contact with coworkers and customers can lead to transmission of SARS-CoV-2 and compromise continuity of operations of workplaces. The Covid-Lab of University of Urbino and the Confindustria Pesaro Urbino association signed an agreement to support the Marche Nord companies in the adoption of anti-contagion safety protocols.
Antibodies detection was performed using a rapid immunochromatographic test. Total RNA from nasopharyngeal swab was subjected to a real-time RT-PCR multiplex for the detection of RdRp specific gene and E gene of the SARS-CoV-2, and the internal control (human RNase P).
Between May 2020 and Apr 2021, over 10,000 rapid serological tests had been carried out on workers of 35 companies and in 5% of cases IgG or IgM were found (519). All the 519 swabs gave a valid result (RNAse P Ct≤40) with 105 positive results (20%) for SARS-CoV-2 with a Ct value ≤45. Overall, only 1% of samples resulted positive for viral RNA (105/10000).
The University of Urbino set up a rapid-response (within 24 h, generally <6 h) diagnostic centre for SARS-CoV-2 detection (Covid-Lab) allowing the companies to activate the optimal safety path to ensure the health and safety of workers in the workplace. Our observations during this first year of activity, highlight that in the workplace, the infection does not seem to spread if precautionary measures are followed and only 1% (1 worker out of 100) tested positive for the SARS-CoV-2 virus.
自 workplace activities involving close contact with coworkers and customers can lead to transmission of SARS-CoV-2 以来，工作场所活动已受到 COVID-19 大流行的深刻影响，这可能导致 SARS-CoV-2 的传播，并影响工作场所的运营连续性。乌比诺大学的 Covid-Lab 与乌尔比诺省和佩斯卡拉省的 Confindustria Pesaro Urbino 协会签署了一项协议，为马尔凯北部的公司提供支持，以采用防传染安全协议。
使用快速免疫层析试验进行抗体检测。从鼻咽拭子提取总 RNA，进行实时 RT-PCR 多重检测，以检测 SARS-CoV-2 的 RdRp 特异性基因和 E 基因，以及内部对照（人 RNase P）。
2020 年 5 月至 2021 年 4 月期间，对 35 家公司的 10,000 多名工人进行了超过 10,000 次快速血清学检测，发现 5%的工人 IgG 或 IgM 呈阳性（519 例）。所有 519 例拭子均获得有效结果（RNAse P Ct≤40），105 例 SARS-CoV-2 检测结果为阳性（20%），Ct 值≤45。总体而言，仅 1%的样本（105/10000）的病毒 RNA 检测结果为阳性。
乌比诺大学建立了一个快速反应（24 小时内，通常<6 小时）SARS-CoV-2 检测诊断中心（Covid-Lab），使公司能够启动最佳安全措施，确保工人在工作场所的健康和安全。在活动的第一年中，我们的观察结果表明，在工作场所，如果采取预防措施，感染似乎不会传播，只有 1%（100 名工人中的 1 名）的 SARS-CoV-2 病毒检测结果为阳性。
