Brown R S, Rubin J R, Rhodes D, Guilley H, Simoncsits A, Brownlee G G
Nucleic Acids Res. 1978 Jan;5(1):23-36. doi: 10.1093/nar/5.1.23.
The nucleotide sequence of tRNATyr from B. stearothermophilus has been determined: pG-G-A-G-G-G-G-s4U-A-G-C-G-A-A-G-U-Gm-G-C-U-A-A-m1A-C-G-C-G-G-C-G-G-A-C-U-Q-U-A-ms2i6A-A-psi-C-C-G-C-U-C-C-C-U-U-U-G-G-G-U-U-C-G-G-C-G-G-T-psi-C-G-A-A-U-C-C-G-U-C-C-C-C-C-U-C-C-A-C-C-AOH. A combination of classical fingerprinting methods, partial nuclease P1 digestion and two-dimensional homochromatography and a rapid "read off" sequencing gel technique were used to establish the complete nucleotide sequence.
嗜热脂肪芽孢杆菌酪氨酸转运核糖核酸(tRNATyr)的核苷酸序列已被确定:pG-G-A-G-G-G-G-s4U-A-G-C-G-A-A-G-U-Gm-G-C-U-A-A-m1A-C-G-C-G-G-C-G-G-A-C-U-Q-U-A-ms2i6A-A-psi-C-C-G-C-U-C-C-C-U-U-U-G-G-G-U-U-C-G-G-C-G-G-T-psi-C-G-A-A-U-C-C-G-U-C-C-C-C-C-U-C-C-A-C-C-AOH。采用经典指纹图谱法、核酸酶P1部分消化法、二维同系层析法和快速“读出”测序凝胶技术相结合的方法确定了完整的核苷酸序列。